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Iranian Journal of Parasitology، جلد ۷، شماره ۴، صفحات ۲۷-۴۰
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عنوان انگلیسی Immunogenicity of MHC Class I Peptides Derived from Leishmania mexicana Gp63 in HLA-A2.1 Transgenic (HHDII) and BALB/C Mouse Models
چکیده انگلیسی مقاله B ackground: L eishmania is an intracellular parasite infecting humans and many wild and domestic animals. Recent studies have suggested an important role for cytotoxic T cells against Leishmania . Peptide-based vaccines targeting short sequences derived from known immunogenic proteins have been shown to elicit cellular immune responses against disparate pathogens. M ethods: We predicted four HLA-A2 peptides derived from L. mexican/major gp63 and tested these in HHD II mice, as well as four peptides for mouse MHC class I from the same proteins tested in BALB/ mice. R esults: The results revealed immunogenicity for three of the four peptides predicted for HLA-A2.Immunisation with these peptides, along with IFA, induced CTL responses detected by standard 4- hour cytotoxicity assay and significantly upregulated the production of IFN-γ. When HHDII mice were injected IM with L. mexicana gp63 cDNA and splenocytes were restimulated with blasts loaded with the immunogenic peptides, two of the peptides were able to induce significant level of IFN-γ detected by ELISA. None of the peptides predicted for Balb/c mouse MHC class I elicited CTL ac- tivity or significantly upregulated the IFN-γ. C onclusion: The results may help in developing a peptide-based vaccine, which can be applied alone or in combination with drugs against Leishmania .
کلیدواژه‌های انگلیسی مقاله HHDII,Peptide,Vaccine,Leishmania
نویسندگان مقاله h رضوان | h rezvan
dept. of laboratory science, school of paraveterinary sciences, bu-ali sina university, hamadan, iran.

سازمان اصلی تایید شده: دانشگاه بوعلی سینا (Bu ali sina university)

r ریز | r rees
school of science and technology, nottingham trent university, clifton, nottingham, uk, ng11 8ns.


علی sa | ali sa
school of science and technology, nottingham trent university, clifton, nottingham, uk, ng11 8ns.
نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/281
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