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Iranian Journal of Parasitology، جلد ۷، شماره ۲، صفحات ۲۹-۳۹
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عنوان انگلیسی Cloning, and Molecular Characterization of Polymorphic Iranian Isolate Theileria annulata Surface Protein (Tasp)
چکیده انگلیسی مقاله Background: Because of the strong immunologic responses of surface protein TaSp in Theileria annu­lata infected host, we tried to characterize this protein in a T. annulata isolate from Iran. Methods: The RNA prepared from T. annulata infected cells was used to produce SMART-DS-cDNA. The Double strand cDNA was then amplified with primers derived from TaSp mRNA se­quences. The PCR product was cloned in pTZ57R/T vector, sequenced and registered under acces­sion no. JQ003240 in GenBank. Results: The sequence analysis showed 90%-94% nucleotide sequence identity and 68%-94% amino acid homology to the corresponding sequences of TaSp gene by T. annulata , T. sp . china I , T. sp . china and T. lestoquardi and three T. annulata reported from Iran respectively. Interestingly, the sequence analysis also showed small nucleotide sequence region near the 5` end in which the presented TaSp protein differed very strongly from the other known TaSp sequences. For the preparation of the recombi­nant protein, the cDNA was cloned in pQE-32 vector, the recombinant protein was pre­pared and assayed by Theileria infected bovine serum. Conclusion: The polymorphism in TaSp gene could be detected in intra- as well as inter species. The different characterized TaSp proteins had a common identic region, which may be helpful for develop­ment of broad band vaccine based on the recombinant proteins. The polymorphism in this gene, make this protein also interesting for the diagnostic purposes.
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نویسندگان مقاله n صدر شیرازی | n sadr shirazi
department of parasitology, iranian center for ticks and tick-borne diseases, faculty of veterinary medicine, university of tehran, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

p شایان | p shayan
department of parasitology, iranian center for ticks and tick-borne diseases, faculty of veterinary medicine, university of tehran, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

b اکرت | b eckert
department of parasitology, iranian center for ticks and tick-borne diseases, faculty of veterinary medicine, university of tehran, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

e ابراهیم زاده | e ebrahimzadeh
department of parasitology, iranian center for ticks and tick-borne diseases, faculty of veterinary medicine, university of tehran, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

n امینی نیا | n amininia
department of parasitology, iranian center for ticks and tick-borne diseases, faculty of veterinary medicine, university of tehran, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/233
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