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Iranian Journal of Parasitology، جلد ۷، شماره ۲، صفحات ۴۰-۴۶
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی Sequence Analysis of the Second Internal Transcribed Spacer (ITS2) Region of rDNA for Species Identification of Trichostrongylus Nematodes Isolated From Domestic Livestock in Iran
چکیده انگلیسی مقاله Background: Infectivity of herbivores with Trichostrongylus nematodes is widespread in many countries, having a major economic impact on breeding, survivability, and productivity of domestic livestock. This study was carried out on Trichostrongylus species isolated from domestic livestock in order to develop an easy-to-perform method for species identification. Methods: Trichostrongylus isolates were collected from sheep, goat, cattle, and buffaloes in Khuzestan Province, southwest Iran. Primary species identification was carried out based on morphological characterization of male worms. PCR amplification of ITS2-rDNA region was performed on genomic DNA and the products were sequenced. Phylogenetic analysis of the nucleotide sequence data was conducted employing Bayesian Inference approach. Consequently, a restriction fragment length polymorphism (RFLP) profile was designed to differentiate Trichostrongylus species. Results: A consensus sequence of 238 nucleotides was deposited in the GenBank for Iranian iso­lates of Trichostrongylus species including T. colubriformis , T. capricola , T. probolurus and T. vitrinus . The designated RFLP using restriction enzyme Tas I could readily differentiate among species having differ­ent ITS2 sequence. The molecular analysis was in concordance with morphological findings. Conclusion: Phylogenetic analysis indicated a close relationship among the sequences obtained in this study and reference sequence of relevant species. ITS2-RFLP with Tas I is recommended for molecu­lar differentiation of common Trichostrongylus species.
کلیدواژه‌های انگلیسی مقاله Trichostrongylus,ITS2,Iran,Livestock
نویسندگان مقاله r قاسمی خواه | r ghasemikhah
vali-e-asr hospital, arak university of medical sciences, arak, iran and department of medical parasitology amp;amp; mycology, school of public health; national institute of health research, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

شربت خور ی | m sharbatkhori
laboratory science research center, golestan university of medical sciences, gorgan, iran and infectious diseases research center, department of medical parasitology and mycology, school of medicine, golestan university of medical sciences, gorgan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی گلستان (Golestan university of medical sciences)

i موبدی | i mobedi
department of medical parasitology amp;amp; mycology, school of public health; national institute of health research, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

eb کیا | eb kia
department of medical parasitology amp;amp; mycology, school of public health; national institute of health research, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

m فصیحی هرندی | m fasihi harandi
department of parasitology, school of medicine, kerman university of medical sciences, kerman, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

h میرهندی | h mirhendi
department of medical parasitology amp;amp; mycology, school of public health; national institute of health research, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/234
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