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Iranian Journal of Parasitology، جلد ۷، شماره ۲، صفحات ۷۳-۸۱
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عنوان انگلیسی Phylogenetic Analysis of Theileria annulata Infected Cell Line S15 Iran Vaccine Strain
چکیده انگلیسی مقاله Background: Bovine theileriosis results from infection with obligate intracellular protozoa of the genus Theileria . The phylogenetic relationships between two isolates of Theileria annulata , and 36 Theile­ria spp . , as well as 6 outgroup including Babesia spp . and coccidian protozoa were analyzed using the 18S rRNA gene sequence. Methods: The target DNA segment was amplified by PCR. The PCR product was used for direct sequencing. The length of the 18S rRNA gene of all Theileria spp . involved in this study was around 1,400 bp. Results: A phylogenetic tree was inferred based on the 18S rRNA gene sequence of the Iran and Iraq isolates, and other species of Theileria available in GenBank. In the constructed tree, Theileria annu­lata (Iran vaccine strain) was closely related to other T. annulata from Europe, Asia, as well as T. lestoquardi , T. parva and T. taurotragi all in one clade. Conclusion: Phylogenetic analyses based on small subunit ribosomal RNA gene suggested that the percent identity of the sequence of Iran vaccine strain was completely the same as Iraq sequence (100% identical), but the similarity of Iran vaccine strain with other T. annulata reported from China, Spain and Italy determined the 97.9 to 99.9% identity.
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نویسندگان مقاله gh حبیبی | gh habibi
department of parasitology, vaccine research and production, razi vaccine and serum research institute, karaj, iran

سازمان اصلی تایید شده: موسسه تحقیقات واکسن و سرم سازی رازی (Razi vaccine and serum research institute)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/239
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