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Iranian Journal of Parasitology، جلد ۷، شماره ۱، صفحات ۲۶-۳۱
عنوان فارسی
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کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Sequence Analysis of Different Domains of Plasmodium vivax Apical Membrane Antigen (PvAMA-1 gene) Locus in Iran
چکیده انگلیسی مقاله Background: Plasmodium vivax is responsible for approximately 80 million malaria cases in the world. Apical membrane antigen1 (AMA-1) is a type I integral membrane protein present in all Plasmo­dium species. AMA-1 interferes in critical steps of invasion of human hepatocytes by sporo­zoites and red blood cells by merozoites and is one of the most immunodominant antigens for elicit­ing a protective immune response in human. It is considered as a promising antigen for inclusion in a vaccine against P. vivax. Since more knowledge is needed to lighten the scope of such antigen we com­pared genetic variation in P. vivax AMA-1from an Iranian isolate with those reported from some of the other malarious countries so far. Methods: P. vivax genomic DNA was extracted from the whole blood of an Iranian patient with pa­tent P. vivax infection. The nucleotide sequence for 446 amino acid (AA) residues (42-488 of PvAMA-1) was amplified by PCR and cloned in pUC19 vector for sequencing. Results: Sequence analysis of the antigen showed a high degree of identity (99%) with strong homol­ogy to the PvAMA-1 gene of P. vivax S3 and SKO814 isolates from India and Korea (Asian isolates) respectively, and 96% similarity with P. vivax Sal-1 AMA-1 gene from El Salvador. Conclusions: We cloned and characterized three domains of PvAMA-1 gene from an Iranian patient. Predicted protein sequence of this gene showed some discrepancies in corresponding protein in compar­ing with similar genes reported from other malarious countries.
کلیدواژه‌های انگلیسی مقاله Plasmodium vivax,Apical membrane antigen,Iran
نویسندگان مقاله a متولی حقی | a motevalli haghi
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

m ناطق پور | m nateghpour
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

غلامحسین ادریسیان | ghh edrissian
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

z sepehrizadeh | z sepehrizadeh
dept. of pharmaceutical biotechnology, pharmacy faculty, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

m محبعلی | m mohebali
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

mr khoramizade | mr khoramizade
dept. of pathobiology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

s صبوری شهربابک | s sabouri shahrbabak
dept. of pharmaceutical biotechnology, pharmacy faculty, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

h مقیمی | h moghimi
dept. of microbiology, faculty of biology, tehran university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/216
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