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Iranian Journal of Parasitology، جلد ۷، شماره ۱، صفحات ۹۷-۱۰۳
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Sequence Diversity in tRNA Gene Locus A-L among Iranian Isolates of Entamoeba dispar
چکیده انگلیسی مقاله Background: A number of methods for detecting diversity in Entamoeba have been described over the years. In the present study the genetic polymorphism of noncoding locus A-L was ana­lyzed using PCR and sequencing in order to clarify the genotypic differences among E. dispar isolates. Methods: A total of 28 E. dispar from patients with gastrointestinal symptoms were determined and the genomic DNA was extracted directly from stool. For genotype analysis; Locus A-L was amplified by PCR and PCR products were sequenced .The sequences obtained were edited ma­nually and aligned using Gene Runner software. Results: With sequencing of PCR products a reliable genetic diversity in size, number and posi­tion of the repeat units were observed among the Iranian E. dispar isolates in locus A-L gene. Se­quences showed variation in length from 448bp to 507bp and seven distinct types were identified. Conclusion: The genetic diversity of loci like A-L shows them to be suitable for epidemiological studies such as the characterization of the routes of transmission of these parasites in Iran.
کلیدواژه‌های انگلیسی مقاله Entamoeba dispar,STRs,Locus A-L,Diversity,Iran
نویسندگان مقاله e احسان مجرد | e nazemalhosseini mojarad
research institute for gastroenterology and liver diseases, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

m عظیمی راد | m azimirad
research institute for gastroenterology and liver diseases, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

z نوچی | z nochi
research institute for gastroenterology and liver diseases, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

s رومانی | s romani
research institute for gastroenterology and liver diseases, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

مهدی تاجبخش ششوان | m tajbakhsh
research institute for gastroenterology and liver diseases, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

m رستمی نژاد | m rostami nejad
research institute for gastroenterology and liver diseases, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

a حقیقی | a haghighi
department of medical parasitology amp;amp; mycology, school of medicine, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

mr زالی | mr zali
research institute for gastroenterology and liver diseases, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/227
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