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Iranian Journal of Parasitology، جلد ۶، شماره ۴، صفحات ۴۱-۴۵
عنوان فارسی
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عنوان انگلیسی Molecular Epidemiology of Cryptosporidiosis in Iranian Children, Tehran, Iran
چکیده انگلیسی مقاله Background: Cryptosporidium is a worldwide protozoan parasite and one of the most common causes of infection and diarrhea in humans and cattle. The aim of the present study was determina­tion of subtypes of Cryptosporidium among children with diarrhea in Tehran by se­quence analysis of the highly polymorphic 60-kDa glycoprotein ( GP60 ) gene. Methods: Fecal samples were collected from 794 diarrheic children. Initial identification of Crypto­spo­ridium was carried out on stool samples by Ziehl-Neelsen acid-fast staining method. DNA was extracted from positive microscopically samples and Cryptosporidium genotypes and subtypes were determined, accordingly. Results: Out of 794 collected samples, 19 (2.40 %) were positive for Cryptosporidium oocysts. Sequences analysis of GP60 gene showed that 17 (89.47 %) of the positive isolates were Crypto­spori­dium parvum and 2 (10.52 %) were C. hominis . All subtypes of C. parvum isolates belonged to allele families IIa (6/17) and IId (11/17). The most common allele in all 17 isolates belonged to IId A20G1a (41.18%). A22G1 (IF) subtype was detected in two C. hominis isolates of the chil­dren. Conclusion: The predominancy of C. parvum species (specially, IId A20G1a sub­type) in current study underlines the importance of zoonotic Cryptosporidium transmission in Iran.
کلیدواژه‌های انگلیسی مقاله Genotypes,Subtypes,Cryptosporidium,GP60 gene,Children,Iran
نویسندگان مقاله n تقی پور | n taghipour
dept. of parasitology, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

e احسان مجرد | e nazemalhosseini mojarad
research institute for gastroenterology and liver diseases, shahid beheshti university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

a حقیقی | a haghighi
dept. of parasitology, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

m رستمی نژاد | m rostami nejad
research institute for gastroenterology and liver diseases, shahid beheshti university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

s رومانی | s romani
research institute for gastroenterology and liver diseases, shahid beheshti university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

a کشاورز | a keshavarz
dept. of parasitology, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

m آل بویه | m alebouyeh
research institute for gastroenterology and liver diseases, shahid beheshti university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

mr زالی | mr zali
research institute for gastroenterology and liver diseases, shahid beheshti university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/203
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