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Iranian Journal of Parasitology، جلد ۶، شماره ۲، صفحات ۱-۸
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عنوان انگلیسی Isolation of Free-Living Amoebae from Sarein Hot Springs in Ardebil Province, Iran
چکیده انگلیسی مقاله Background: Free-living amoebae (FLA) are a group of ubiquitous protozoan, which are distrib­uted in the natural and artificial environment sources. The main aim of the current study was to identify the presence of FLA in the recreational hot springs of Sarein in Ardebil Province of Iran. Methods: Seven recreational hot springs were selected in Sarein City and 28 water samples (four from each hot spring) were collected using 500 ml sterile plastic bottles during three month. Filtra­tion of water samples was performed, and culture was done in non-nutrient agar medium enriched with Escherichia coli . Identification of the FLA was based on morphological criteria of cysts and trophozoites. Genotype identification of Acanthamoeba positive samples were also per­formed using sequencing based method. Results: Overall, 12 out of 28 (42.9%) samples were positive for FLA which Acanthamoeba and Vahlkampfiid amoebae were found in one (3.6%) and 11 (39.3%) samples, respectively. Se­quence analysis of the single isolate of Acanthamoeba revealed potentially pathogenic T 4 geno­type corresponding to A. castellanii . Conclusion: Contamination of hot springs to FLA, such as Acanthamoeba T 4 genotype (A. castel­lanii) and Vahlkampfiid amoebae, could present a sanitary risk for high risk people, and health authorities must be aware of FLA presence.
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نویسندگان مقاله a بدیرزاده | a badirzadeh
department of parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

m نیتی | m niyyati
department of parasitology and mycology, school of medicine, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

z بابایی | z babaei
department of parasitology and mycology, faculty of medicine, kerman university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

امینی امیرکلایی | h amini
department of environmental health engineering, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

h بدیرزاده | h badirzadeh
laboratory of microbiology and parasitology, school of medicine, ardebil university of medical sciences, ardebil, iran


محمد رضاییان | m rezaeian
department of parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/172
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