این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Parasitology، جلد ۶، شماره ۱، صفحات ۸۶-۹۱
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Haemolymph Components of Infected & None Infected Lymnaea snails with Xiphidiocercariae
چکیده انگلیسی مقاله Background: In this study the haemolymph components of infected and none infected Lymnaea gedrosiana with xiphidiocercaria larvae was compared. Methods: Five hundred Fifty Lymnaea snails were collected from Ilam and Mazandaran prov­inces, Iran, during 2008-2009. The snails were transported to the lab at Tehran University of Medi­cal Sciences and their cercarial sheddings were studied. Haemolmyphs of snails were ex­tracted and cells were counted using haemocytometer and cell-surface carbohydrate were recog­nized by conjugated lectin (Lentil). Haemolymph protein concentrations were measured by Brad­ford protein assay method and soluble protein compositions were determined on sodium dodecyl sulphate polyacrilamide gel electrophoresis (SDS-PAGE). Result: From the 550 examined Lymnaea snails for cercariae, 27 snails were infected with xiphidiocer­cariae. Mean of haemolymph cells (haemocyte) number were obtained 93480±2.43 (cells/ml) for none infected snails (25 snail) and 124560±2800 (cells/ml) for infected snails (25 snail). Mannose carbohydrate was recognized on haemocyte of none infected and infected snails. Mean of protein concentration of haemolymph plasma was obtained as 1354 ± 160 μg/ml (1.4 mg/ml) for none infected snails (25 snails) and 1802±138 μg/ml (1.8 mg/ml) for infected snail (25 snails). Comparing to none infected snails, the SDS-PAGE results of haemolymph plasma of infected snails, showed an extra protein band (70 kDa). The results showed a significant differ­ence between the amounts and the kinds of proteins in haemolymph of infected and none infected snails. Conclusion: This information might be useful to understand of parasite detection, adhesion, engulf­ment and antigen agglutination by snail.
کلیدواژه‌های انگلیسی مقاله Cercaria,Lymnaea,Haemolymph,Xiphidiocercariae
نویسندگان مقاله aa صبور یراقی | aa saboor yaraghi
dept. of nutrition and biochemistry, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

a فرحناک | a farahnak
dept. of parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

mr اشراقیان | mr eshraghian
dept. of epidemiology and biostatistics, school of public health, tehran university of medical sciences, tehran, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/170
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات