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Iranian Journal of Parasitology، جلد ۵، شماره ۱، صفحات ۲۳-۳۴
عنوان فارسی
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عنوان انگلیسی Mini-Exon Genotyping of Leishmania Species in Khuzestan Province, Southwest Iran
چکیده انگلیسی مقاله Background: Leishmaniasis is a protozoan disease cause by Leishmania genus. Anthroponotic and zoono­tic cutaneous leishmaniasis are endemic in Iran. The aim of this study was to identify the causative agent of cutaneous leishmaniasis by mini-exon gene in five regions of Khuzestan Province, southwest of Iran. Methods: From 2007 to 2008 in this cross-sectional study, cutaneous samples were collected from patients referred to Health Centers and Hospitals of the Khuzestan Province for cutaneous leishmaniasis diagnosis and cultured in Novy-MacNeal-Nicolle (NNN) and RPMI 1640. The propagated promastigotes were harvested and Leishmania species of cutaneous leishmaniasis were identified by RFLP and DNA sequencing of the PCR generated fragments. Results: L. major and L. tropica were the causative agents of cutaneous leishmaniasis by predomi­nantly of L. major species. The alignment of the mini-exon sequencing isolates with re­ported sequencing of L. major and L. tropica revealed 92%-99% identity. Conclusion: Our study showed that mini-exon PCR-RFLP was useful method to identify the causa­tive species of cutaneous leishmaniasis.
کلیدواژه‌های انگلیسی مقاله Cutaneous leishmaniasis,Mmini-exon,RFLP,Sequencing,Iran
نویسندگان مقاله j ساکی | j saki
department of medical parasitology and mycology, faculty of medicine, iran university of medical sciences, tehran, iran and department of medical parasitology and mycology, faculty of medicine, ahvaz jundishapur university of medical sciences, ahvaz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (Ahvaz jundishapur university of medical sciences)

ar معمار | ar meamar
department of medical parasitology and mycology, faculty of medicine, iran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

h اورمزدی | h oormazdi
department of medical parasitology and mycology, faculty of medicine, iran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

l اخلاقی | l akhlaghi
department of medical parasitology and mycology, faculty of medicine, iran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

s مراغی | s maraghi
department of medical parasitology and mycology, faculty of medicine, ahvaz jundishapur university of medical sciences, ahvaz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (Ahvaz jundishapur university of medical sciences)

m محبعلی | m mohebali
department of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

s خادم وطن | s khademvatan
department of medical parasitology and mycology, faculty of medicine, ahvaz jundishapur university of medical sciences, ahvaz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (Ahvaz jundishapur university of medical sciences)

e رزمجو | e razmjou
department of medical parasitology and mycology, faculty of medicine, iran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/123
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