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Iranian Journal of Parasitology، جلد ۴، شماره ۳، صفحات ۱-۹
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عنوان انگلیسی Gene Cloning of Iranian Leishmania major Mannose-1-Phosphate Guanyltransferase
چکیده انگلیسی مقاله Background: Leishmania is an obligatory intracellular protozoan parasite, which infects human be­ings when infected sand fly vector takes a blood meal. Most efforts are towards designing an effective vaccine to prevent leishmaniasis. In this way, development of candidate antigen for vaccine has spe­cial im­portant. In this study, we cloned mannose-1-phosphate guanyltransferase gene of Iranian L .major in pET32a expression vector. Methods: Primers based on L. major mannose-1-phosphate guanyltransferase sequence gene was de­signed and synthesized. DNA of Leishmania promastigotes was extracted and PCR reaction was done. PCR product was cloned into pTZ57R and sub cloned into pET32a expression vector. Results: Recombinant plasmid containing 1140 bp as L. major mannose-1-phosphate guanyltrans­ferase gene was extracted and confirmed by restriction analysis. PCR product was sequenced and de­posited to GenBank. There were some differences in amino acid sequences between Iranian L. major mannose-1-phosphate guanyltransferase and others previously accepted in GenBank Conclusion: We amplified and cloned Iranian L. major mannose-1-phosphate guanyltransferase suc­cessfully.
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نویسندگان مقاله sh حجازی | sh hejazi


n ضیای جهرمی | n zia jahromi


m بنده پور | m bandehpour


g اسلامی | g eslami


r صالحی | r salehi


a خامسی پور | a khamesipour


b کاظمی | b kazemi


نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/102
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