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Iranian Journal of Parasitology، جلد ۴، شماره ۲، صفحات ۲۳-۳۱
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عنوان انگلیسی Induction of Apoptosis by Miltefosine in Iranian Strain of Leishmania infantum Promastigotes
چکیده انگلیسی مقاله Background : Miltefosine is a new drug of choice for the treatment of visceral leishmaniasis. Numerous experi­mental studies have shown miltefosine is effective on Leishmania donovani , however, effectiveness of miltefos­ine in treatment of L. infantum is not fully understood. The aims of the present study were to evaluate cytotoxic effects of miltefosine on Iranian strain of L. infantum , and to determine its 50% inhibitory concentration (IC50) as well as lethal dose. Methods : Anti- L. infantum activity of miltefosine was studied by treatment of cultured promastigotes with vari­ous concentration of miltefosine. MTT assay was used to determine L. infantum viability and the results were expressed as IC50. Annexin-V FLUOS staining was performed to study apoptotic properties of this drug by us­ing FACS flow cytometry. Results : Miltefosine led to dose-dependent death of L. infantum with features compatible with apoptosis includ­ing cell shrinkage, DNA laddering, and externalization of phosphatidylserine with preservation of integrity of plasma membrane . The 100% effect was achieved at 22 µM and IC50 after 48 hours of incubation was 7 μM. Conclusion : Miltefosine exerts cytotoxic effect on Iranian strain of L. infantum via an apoptosis-related mecha­nism.
کلیدواژه‌های انگلیسی مقاله Leishmania infantum, Miltefosine, IC50, Apoptosis, Iran
نویسندگان مقاله s خادم وطن | s khademvatan


mj غروی | mj gharavi


l اخلاقی | l akhlaghi


a صمدی کوچکسرایی | a samadikuchaksaraei


k موسوی زاده | k mousavizadeh


r حدیقی | r hadighi


j ساکی | j saki


نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/94
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