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Iranian Journal of Parasitology، جلد ۴، شماره ۲، صفحات ۳۲-۳۷
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عنوان انگلیسی Determination of Diagnostic Antigens in Cattle Amphistomiasis Using Western Blotting
چکیده انگلیسی مقاله Background: Mixed infection with amphistomes seems common in native cattle of Iran. The aim of this study was to determine diagnostic antigens in cattle mixed amphistomiasis. Methods: Specific antigens of Cotylophoron cotylophorum , Gastrothylax crumenifer and Paramphisto­mum cervi (mixed infection), the most common species, were collected from cattle was deter­mined. Adult trematodes were collected from the rumen of naturally infected cattle at meat inspec­tion. After their homogenization and centrifugation, somatic antigens were prepared and ana­lyzed by SDS-PAGE. Specific antigens were determinated by western blot with homologous and heterolo­gous sera. SDS-PAGE of whole worms extract was performed at different concentrations and subse­quent gels staining. Immunoblotting analysis using sera from cattle naturally infected with am­phistomes, Dicrocoelium dendriticum , Fasciola spp. and hydatid cyst was performed. Results: Electrophorese analysis of somatic antigens revealed the presence of 10 and 21 protein bands at 4 µgr/ml and 8 µgr/ml with molecular weights ranging from 25-120 and 25-150 kDa, respectively. The best result was taken at 8 mg/ml concentration. Although western blot of these proteins demon­strate 5 major antigenic polypeptides ranging from 50 to 100 kDa which were recognized by serum of cat­tle naturally infected with mixed amphistomes.
کلیدواژه‌های انگلیسی مقاله Amphistomiasis, Cattle, Antigens, Western bloting
نویسندگان مقاله b مشگی | b meshgi


ابوالفضل اسلامی | a eslami


a حلاجیان | a halajian


نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/95
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