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Iranian Journal of Parasitology، جلد ۴، شماره ۱، صفحات ۴۰-۴۷
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عنوان انگلیسی The Effect of Alkanna tincturia and Peganum harmala Extracts on Leishmania major (MRHO/IR/75/ER) in vitro
چکیده انگلیسی مقاله Background: Cutaneous leishmaniasis is an important health problem caused by Leishmania spp . As there is no vaccine, drug treatment is the only way to tackle leishmaniasis. In the present study, inhibitory and kill­ing effects of Peganum harmala and Alkana tinctoria extracts on amastigotes and promastigotes forms of Leishmania were evaluated in-vitro. Methods: The seeds of Peganum harmala, Stems and roots of Alkanna tictoria were collected and crude extrac­tion carried out. In this experimental study, Leishmania major promastigotes were cultured in RPMI-1640 with 10% FBS at 22-26°C, and infected macrophages with amastigotes were cultured in RPMI-1640 with 10% FBS at 37°C in 5% CO2. Then the extracts of each plant were added to cultivated parasites and incubated for 3 days. Promastigote and amastigote assay was carried out using counting assay based on growth inhibition. Results: The results indicated that both extractions can inhibit the growth of promastigotes, and in concentra­tions of 40µg/ml of P. harmala , 200µg/ml of A. tincturia, and 20 µg/ml of equal combination of P. hamala and A. tincturia are Inhibitory Concentration (IC 50 ) for parasites growth. By adding these concentra­tions of the extracts to the infected macrophages in the culture, their effects were separately eva­lu­ated. The mean of amastigotes number in macrophages in the culture with P. harmala, A. ticturia , combina­tion and control groups were 0.7, 0.7, 0.6, 2.3 amastigotes per macrophage, respectively. Conclusion: By this method, inhibition of intracellular and extracellular growth of L. major was demon­strated suggesting that, plant drugs with efficacy and safe products can be applied as new treatment for cutane­ous leishmaniasis.
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نویسندگان مقاله روح الله یوسفی | ruhallah yousefi
dept. of parasitology, school of medicine, tarbiat modarres university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

فاطمه غفاری فر | fatemeh ghaffarifar


عبدالحسین دلیمی اصل | abdolhosein dalimi asl


نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/88
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