این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Parasitology، جلد ۳، شماره ۴، صفحات ۳۹-۴۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Variation of the Chloroquine Resistance Transporter (Crt) Gene in Chloroquine-Resistant and Chloroquine-Sensitive Plasmodium berghei
چکیده انگلیسی مقاله Background: The emergence and spread of chloroquine resistant Plasmodium falciparum in the world stimulated some investigators to consider different aspects of chloroquine resistance in human and rodent Plasmodia . Using animal Plasmo­dia , particularly primate and rodent Plasmodia can be useful model for human Plasmodia studies. In this study we have tried to consider and compare the sequence of chloroquine resistance transporter ( crt ) gene among chloroquine-resistant and chloroquine-sensitive strains of Plasmodium berghei . Methods: This experimental study was performed at the Malaria Laboratory of School of public health. DNA was ex­tracted from two strains of P. berghei which their resistance and sensitivity had been demonstrated in mice with treatment by chloroquine. By using specific primer for crt gene some parts of this gene were amplified by PCR, and obtained frag­ments were then sequenced and compared. Results: There were considerable differences in crt gene between two strains. Sequenced 1212 bp of crt gene fragment in the two strains showed 43 differences at nucleotides level and 16 differences in presumed coding amino acids. Conclusion: crt can be addressed as a considerable gene which involves in induction of resistance to chloroquine in P. berghei , as P. falciparum . The results increased such a promise that considering crt gene in chloroquine-sensitive and chlo­roquine-resistant P. berghei can prepare suitable and helpful fields for more understanding the molecular aspects of chloro­quine-resistance in Plasmodia and reversing the effectiveness of 4-aminoquinolines particularly chloroquine for treatment of drug resistant Plasmodia .
کلیدواژه‌های انگلیسی مقاله Chloroquine resistance, Plasmodium berghei, Crt gene
نویسندگان مقاله n قباخلو | n ghobakhloo
dept. of medical parasitology amp;amp; mycology, school of public health and institute of public health res


m ناطق پور | m nateghpour
dept. of medical parasitology amp;amp; mycology, school of public health and institute of public health res


رضایی | s rezaee
dept. of medical parasitology amp;amp; mycology, school of public health and institute of public health res


h حجاران | h hajjaran
dept. of medical parasitology amp;amp; mycology, school of public health and institute of public health res


m محبعلی | m mohebali
dept. of medical parasitology amp;amp; mycology, school of public health and institute of public health res


h عابد خجسته | h abedkhojasteh
dept. of medical parasitology amp;amp; mycology, school of public health and institute of public health res
نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/78
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات