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Iranian Journal of Parasitology، جلد ۳، شماره ۳، صفحات ۳۰-۳۶
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عنوان انگلیسی Genetic Characterization of Cryptosporidium spp. among Children with Diarrhea in Tehran and Qazvin Provinces, Iran
چکیده انگلیسی مقاله Background : Cryptosporidium is an intracellular apicomplexan parasite that infects a wide range of vertebrates including humans. Cryptosporidiosis is a major cause of diarrhea in children with and without human immunodeficiency virus (HIV) infection in developing countries. More recently, the molecular methods for identification of morphologically indistin­guishable species have been developed. The aim of this study was to determine the characterization of various species of this coccidian among children with diarrhea by using molecular methods. Methods : Fecal samples were collected from 1263 children with diarrhea who referred to Pediatrics Medical Centers in Qazvin and Tehran, two central provinces of Iran. Initial identification of Cryptosporidium was carried out by Zeihl-Neel­sen acid-fast staining method of stool samples. DNA was extracted from positive microscopically samples and were sub­jected to a two step nested PCR-RFLP based on SSU-rRNA gene. Results : Out of 1263 collected samples, 31 (2.5%) were found to be contained Cryptosporidium oocysts. RFLP analysis showed that 80.6% of the positive isolates were Cryptosporidium parvum, 16.1% C. hominis and 3.2% had mix infection pattern of both C . parvum and C. hominis . Conclusion : Our results showed that the zoonotic pattern of transmission is predominant and has considerable significance in epidemiology of cryptosporidiosis in the study areas.
کلیدواژه‌های انگلیسی مقاله Cryptosporidum, Genotyping, SSU-rRNA, Children, Diarrhea
نویسندگان مقاله a کشاورز | a keshavarz
dept. of parasitology, shahid beheshti university, m. c., tehran, iran

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

a اطهری | a athari
dept. of parasitology, shahid beheshti university, m. c., tehran, iran

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

a حقیقی | a haghighi
dept. of parasitology, shahid beheshti university, m. c., tehran, iran

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

b کاظمی | b kazami
dept. of parasitology, shahid beheshti university, m. c., tehran, iran

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

a آبادی | a abadi
dept. of community amp;amp; health, shahid beheshti university, m. c. tehran, iran

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

e nazemalhoseini مجرد | e nazemalhoseini mojarad
research center for gastroenterology and liver diseases, shahid beheshti university, m. c. tehran, i

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

l کاشی | l kashi
pediatrics medical center, tehran university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/68
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