این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Veterinary Science and Technology، جلد ۴، شماره ۱، صفحات ۲۹-۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Isolation and identification of Brachyspira pilosicoli from laying hens flocks, using conventional culture and molecular methods in Mashhad, Iran
چکیده انگلیسی مقاله Avian intestinal spirochaetosis (AIS) is an intestinal infection caused by anaerobic spirochaetes of the genus Brachyspira, including B. pilosocoli. The purpose of this study was isolation and identification of B. pilosocoli from laying hen flocks, located in Mashhad suburb, Khorasan Razavi province, Iran, and investigating the frequency of the infection. One hundred and eighty cloacal swab samples from 18 randomly selected flocks (10 samples /flock) were cultured anaerobically on selective agar and confirmed as intestinal spirochaete by its spirichaetal form using phase contrast microscopy. Then, the samples were subjected to PCR amplification followed by DNA sequencing. A total of 24 samples from 8 flocks were selected as suspected cases by culture and phase contrast microscopy. Upon PCR amplification by specific primers, only 9 cultures belonged to 3 flocks appeared to be B.pilosicoli. Sequence analysis of the amplicons confirmed the identity of all isolated ones. Based on the results obtained, it was concluded that B. pilosicoli might be strongly involved in AIS among laying hen flocks of this geographical region. The results could also be considered as an indicator for large scale investigation into the true prevalence of the infection. This study is the first report of infection in laying hens flocks of Iran.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله محمدرضا باسامی | mohammad reza bassami


عبدالله جمشیدی | abdollah jamshidi


آیدا کسایی کسایی | aida kasaei kasaei


اعظم محمدی محمدی | azam mohamadi mohamadi


نشانی اینترنتی http://ijvst.um.ac.ir/index.php/veterinary/article/view/16765
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/468/article-468-393558.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات