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Journal of Arthropod-Borne Diseases، جلد ۱۰، شماره ۴، صفحات ۵۴۶-۰
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عنوان انگلیسی Genetic Analysis of Aedes aegypti using Random Amplified Polymorphic DNA (RAPD) Markers from Dengue Outbreaks in Pakistan
چکیده انگلیسی مقاله Background: Keeping in view the havoc situation of dengue fever in Pakistan, the current study was designed to demon­strate the genetic variations, gene flow and rate of migration from Lahore and Faisalabad. Methods: The larvae were collected from both natural and artificial breeding places from each collection site. The adult mosquitoes were collected by means of sweep net and battery-operated aspirator. DNA extraction was per­formed using TNE buffer method. Ten GeneLink-A series RAPD primers were used for PCR amplification and the data was analyzed through POPGENE. Results: The number of amplification products produced per primer varied from 8-12, ranging from 200 to 2000 bp with an average of 10.0 bands per primer. The percentage of polymorphic loci amplified by each primer varied from 22.5 to 51%. The UPGMA dendrogram demonstrates two distinct groups from Faisalabad and Lahore populations. The genetic diversity ranged from 0.260 in Faisalabad to 0.294 in Lahore with a total heterozygosity of 0.379. The G ST value for nine populations within Lahore was 0.131 (Nm= 3.317), whereas for nine populations in Faisalabad G ST value was 0.117 (Nm= 3.773). The overall genetic variation among eighteen populations showed G ST = 0.341 and Nm= 1.966. Conclusion: The genetic relatedness and Nm value show that Ae . aegypti populations exhibit intra-population gene flow both in Faisalabad and Lahore. Although, both cities show a distinct pattern of genetic structure; however, few areas from both the cities show genetic similarity. The gene flow and the genetic relatedness in few populations of Lahore and Faisalabad cities need further investigation.
کلیدواژه‌های انگلیسی مقاله Aedes aegypti, Dengue, Genetic diversity, RAPD markers
نویسندگان مقاله حفیظ محمد اشرف | hafiz muhammad ashraf
department of zoology, government college university faisalabad, pakistan


محمد kashif زهور | muhammad kashif zahoor
department of zoology, government college university faisalabad, pakistan


شباب نصیر | shabab nasir
department of zoology, government college university faisalabad, pakistan


humara ناز مجید | humara naz majeed
department pharmaceutical and biological chemistry, school of pharmacy, university college london, uk


sarwat زهور | sarwat zahoor
centre of agricultural biochemistry and biotechnology cabb , university of agriculture faisalabad, pakistan
نشانی اینترنتی http://jad.tums.ac.ir/index.php/jad/article/view/344
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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