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International Journal of Hematology-Oncology and Stem Cell Research، جلد ۲۰۱۵، شماره ۱۰، صفحات ۰-۰
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عنوان انگلیسی The Impact of Confluence on BMMSC Proliferation and Osteogenic Differentiation".
چکیده انگلیسی مقاله Background: In the field of cellular therapy, the impact of confluence degree to harvest or differentiate BMMSCs and the effect of cell-to-cell contact remains controversial. Therefore, the effect of confluence on BMMSCs properties was studied and confluence-associated osteogenic differentiation efficiency was identified. Materials and Methods: The impact of confluences at 20, 50, 70, 80 and 100% on BMMSCs proliferation properties, expression of ERK and p-ERK proteins and glucose consumption rate was studied. Confluence-associated osteogenic differentiation efficiency was identified by determining calcium deposition, alizarin red staining, ALP activity and osteopontin and osteocalcin genes expression. Results: There was a correlation between confluence % and the BMMSCs density. Viability was declined at the lower and higher confluences. The highest CFU-F, Brd-U uptake and population doubling were obtained at 80% confluence. ERK band intensity in 100% confluent BMMSCs was lower compared to other confluences. Bands of p-ERK were highly detectable in 70% and 80% confluences. Glucose consumption rate at 70% and 80% confluences in the last days were higher than at 20% and 100% confluences. Although higher osteogenic differentiation estimated by calcium deposition, alizarin red stain and ALP activity at 80% confluences, it was also extended at 100% confluence. Osteopontin gene expressed among all confluences including 100% while osteocalcin gene was expressed highly in 70% confluent cells. Conclusion: We concluded that the optimum seeding density for maximal expansion and harvesting purposes is 80% confluence and up to 100% confluence for osteogenic differentiation to trigger the process to be more cost effective.
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نویسندگان مقاله faten ابو عزیزه محمد ابو عزیزه | faten abo aziza mohamed abo aziza
national research centre


عبد el kader احمد زکی | abd el kader ahmed zaki
cairo university
نشانی اینترنتی http://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/592
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