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International Journal of Hematology-Oncology and Stem Cell Research، جلد ۹، شماره ۱، صفحات ۱-۴
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عنوان انگلیسی Presence of both Mesenchymal and Carcinomatous Features in an In-vitro Model of Ovarian Carcinosarcoma Derived from Patients' Ascitic Fluid.
چکیده انگلیسی مقاله We have refined the technique for isolating and propagating cultures of primary ovarian carcinosarcoma cells (OSCs) derived from ascites, which allowed the cells to obtain the biphasic features of carcinosarcoma in cell culture conditions (presence of both carcinoma and mesenchymal morphologic types). This protocol involves a simple yet rapid method for the growth and propagation of ascites OSC in a basal culture medium. Autologous ascitic fluid was used as source of growth factors, and minimal manipulation was involved to establish the culture. The methodology allowed for the direct application of multiple molecular, cellular, and functional analyses within a few weeks of initial cell isolation, with the further potential of retrospective analyses of archived cells and tissues.
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نویسندگان مقاله احمد شریف تبریزی | ahmad shariftabrizi
department of pathology, tufts university school of medicine, boston, ma 02111, usa.


ilenia pellicciotta | ilenia pellicciotta
department of radiation oncology, new york university college of medicine, new york, ny 10016, usa.


عامر عبدالله | amer abdullah
department of obstetrics, gynecology and reproductive biology, danbury hospital, 24 hospital avenue, danbury, ct 06810, usa.


charis anne venditti | charis anne venditti
department of obstetrics, gynecology and reproductive biology, danbury hospital, 24 hospital avenue, danbury, ct 06810, usa.


رابرت samuelson | robert samuelson
department of obstetrics, gynecology and reproductive biology, danbury hospital, 24 hospital avenue, danbury, ct 06810, usa.


شهره شهابی | shohreh shahabi
department of obstetrics, gynecology and reproductive biology, danbury hospital, 24 hospital avenue, danbury, ct 06810, usa.
نشانی اینترنتی http://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/402
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