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Iranian Journal of Parasitology، جلد ۱۲، شماره ۲، صفحات ۲۰۶-۲۱۴
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عنوان انگلیسی Detection of Potentially Diagnostic Leishmania Antigens with Western Blot Analysis of Sera from Patients with Cutaneous and Visceral Leishmaniases
چکیده انگلیسی مقاله Background: Visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL) are important public health problems in Iran. We aimed to evaluate the diagnostic potential of Western blot (WB) compared with indirect immunofluorescence test (IFAT) to serodiagnosis of leishmaniasis. Methods: This study was performed from 2010-2014 and participants were different parts of Iran. Serum samples were obtained from 43 patients with proven CL, 33 patients with proven VL, 39 patients with other parasitic diseases and 23 healthy individuals. Results: WB sensitivity for CL and VL was 100% and 91%, compared to IFA 4.6% and 87.8%, respectively. Sera from patients with CL and VL recognized numerous antigens with molecular weights ranging from 14 to 68 kDa and 12 to 94 kDa, respectively. The most sensitive antigens were 14 and 16 kDa for CL recognized by 100% of the sera from patients with proven CL and 12, 14 and 16 kDa for VL, recognized by 63.6%, 100% and 63.6% of the sera from patients with proven VL respectively. WB analysis is more sensitive than IFAT for the diagnosis of leishmaniasis particularly in cases of cutaneous leishmaniasis. The 12, 14 and 16 kDa can be valuable diagnostic molecules for serodiagnosis of leishmaniasis because at least two immunogenic molecules were simultaneously detected by all patient sera, as well as produced antibodies against these antigens have no cross-reactivity with other control groups. Conclusion: WB could be useful for screening and serodiagnosis of CL and VL in epidemiologic studies in endemic areas.
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نویسندگان مقاله سید جواد سید طبایی | seyyed javad seyyedtabaei
dept. of parasitology and mycology, school of medicine, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

علی رستمی | ali rostami
dept. of parasitology and mycology, school of medicine, shahid beheshti university of medical sciences, tehran, iran student research committee, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

علی حقیقی | ali haghighi
dept. of parasitology and mycology, school of medicine, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

مهدی محبعلی | mehdi mohebali
dept. of medical parasitology amp;amp; mycology, school of public health, tehran university of medical sciences, tehran, iran center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

بهرام کاظمی | bahram kazemi
dept. of biotechnology, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

شیرزاد فلاحی | shirzad fallahi
dept. of parasitology and mycology, school of medicine, lorestan university of medical sciences, khorramabad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی لرستان (Lorestan university of medical sciences)

عادل اسپوتین | adel spotin
dept. of parasitology and mycology, school of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1627
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-403711.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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