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Iranian Journal of Parasitology، جلد ۱۲، شماره ۲، صفحات ۲۹۲-۲۹۷
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عنوان انگلیسی Evaluation of Microsatellites Markers to Discriminate Four Main Taeniid Tapeworms of Dogs
چکیده انگلیسی مقاله Background: Echinococcus granulosus, Taenia multiceps, Taenia ovis and Taenia hydatigena are among the most prevalent taeniid species of dogs. These tapeworms infect ruminant and humans as intermediate hosts and domestic/wild carnivores as the definitive hosts. Molecular tools using hypervariable microsatellite regions might provide more information about parasite variation. Highly variable and specific tools are needed for transmission tracking studies of canine echinococcosis as an essential element for implementation of hydatid control programs. Suitable microsatellite markers used so far are EmsJ, EmsK, EmsB, EMms1, Egmsca1, Egmsga1, U1 snRNA. The purpose of the present study was to determine the microsatellite variability of EmsB as well as six other microsatellites in major taeniid species infecting dogs in Iran. Methods: Twenty isolates of each of the four Taeniidae tapeworms were collected from sheep during routine veterinary inspection in Tehran, Alborz and Kerman provinces from October 2010 to May 2011. After DNA extraction, PCR was set up with optimum conditions using specific primers for each individual microsatellite marker. All the PCR products were evaluated by agarose gel electrophoresis. We used SDS-PAGE for evaluating patterns of PCR products in the tapeworms. Results: E. granulosus as well as Taenia species could be differentiated based on EmsB microsatellite patterns. The electrophoresis patterns of two taeniid genera were readily distinguishable. EmsB could be specifically used in epidemiological studies of canine echinococcosis. Conclusion: Different patterns of EmsB proved this microsatellite marker as a reliable tool for epidemiological studies on canine echinococcosis.
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نویسندگان مقاله سعیده شمس الدینی | saeedeh shamsaddini
research center for hydatid disease in iran, school of medicine, kerman university of medical sciences, kerman, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

محمد علی محمدی | mohammad ali mohammadi
dept. of medical laboratory sciences, sirjan school of medical sciences, kerman university of medical sciences, ker-man, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

سید رضا mirbadie | seyed reza mirbadie
dept. of basic medical sciences, school of medicine, shahroud university of medical sciences, shahroud, iran


سعید نصیبی | saeid nasibi
research center for hydatid disease in iran, school of medicine, kerman university of medical sciences, kerman, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

سیما رستمی | sima rostami
dept. of research and technology, alborz university of medical sciences, karaj, iran


منصوره دهقانی | mansoureh dehghani
research center for hydatid disease in iran, school of medicine, kerman university of medical sciences, kerman, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

مجید فصیحی هرندی | majid fasihi harandi
research center for hydatid disease in iran, school of medicine, kerman university of medical sciences, kerman, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1636
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-403725.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Short Communication(s)
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