این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Parasitology، جلد ۱۲، شماره ۳، صفحات ۳۳۲-۳۳۸
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Sequence Variation in Rhoptry Neck Protein 10 Gene among Toxoplasma gondii Isolates from Different Hosts and Geographical Locations
چکیده انگلیسی مقاله Background: Toxoplasma gondii , as a eukaryotic parasite of the phylum Apicomplexa, can infect almost all the warm-blooded animals and humans, causing toxoplasmosis. Rhoptry neck proteins (RONs) play a key role in the invasion process of T. gondii and are potential vaccine candidate molecules against toxoplasmosis. Methods: The present study examined sequence variation in the rhoptry neck protein 10 (TgRON10) gene among 10 T. gondii isolates from different hosts and geographical locations from Lanzhou province during 2014, and compared with the corresponding sequences of strains ME49 and VEG obtained from the ToxoDB database, using polymerase chain reaction (PCR) amplification, sequence analysis, and phylogenetic reconstruction by Bayesian inference (BI) and maximum parsimony (MP). Results: Analysis of all the 12 TgRON10 genomic and cDNA sequences revealed 7 exons and 6 introns in the TgRON10 gDNA. The complete genomic sequence of the TgRON10 gene ranged from 4759 bp to 4763 bp, and sequence variation was 0-0.6% among the 12 T. gondii isolates, indicating a low sequence variation in TgRON10 gene. Phylogenetic analysis of TgRON10 sequences showed that the cluster of the 12 T. gondii isolates was not completely consistent with their respective genotypes. Conclusion: TgRON10 gene is not a suitable genetic marker for the differentiation of T. gondii isolates from different hosts and geographical locations, but may represent a potential vaccine candidate against toxoplasmosis, worth further studies.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله yu zhao | yu zhao
state key laboratory of veterinary etiological biology, key laboratory of veterinary parasitology of gansu prov-ince, lanzhou veterinary research institute, chinese academy of agricultural sciences, lanzhou, china


donghui zhou | donghui zhou
state key laboratory of veterinary etiological biology, key laboratory of veterinary parasitology of gansu prov-ince, lanzhou veterinary research institute, chinese academy of agricultural sciences, lanzhou, china


جیا chen | jia chen
college of veterinary medicine, gansu agricultural university, lanzhou, china


xiaolin سون | xiaolin sun


نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1763
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-429497.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article(s)
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات