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Iranian Journal of Parasitology، جلد ۱۲، شماره ۳، صفحات ۳۹۸-۴۰۷
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عنوان انگلیسی The Anti-leishmanial Efficacy of Artemisia dracunculus Ethanolic Extract in Vitro and Its Effects on IFN- γ and IL-4 Response
چکیده انگلیسی مقاله Background: Leishmaniasis is a parasitic disease that appears with a range of symptoms including cutaneous, mucocutaneous, and visceral leishmaniasis. The present study sought to determine the antileishmanial effect of the extract of Artemisia dracunculus (Tarragon) compared to control treatment with pentavalent antimony (meglumine). Methods: This experimental study was performed in 2014-2015. A. dracunculus were collected from West Azerbaijan Province, Iran and dried; then the ethanolic extract of the plant was prepared. The effect of different concentrations of Artemisia's extract was compared with Glucantime ® in the stationary phase by MTT colorimetric assay and Trypan blue staining. Human peripheral blood mononuclear cells (HPBMCs) treated with L. major and production of IFN- γ and IL-4 cytokines measured at concentrations of 25, 20, 10 and 5µg/ml A. dracunculus . Results: Treatment with the extract did not affect the survival of the parasites during the first 48 h; however, on the third day (72 h), all concentrations significantly reduced the number of parasites with an efficacy of more than 50% at 10 µg/ml ( P < 0.01), 20µg/ml ( P < 0.001), and 25 µg/ml ( P < 0.0001). Moreover, IFN-γ and IL-4 secretion from the HPBMCs was significantly affected in a dose-dependent manner, compared to the control (no extract). The IFN-γ/IL-4 ratio further confirmed this notion. Conclusion: A. dracunculus extract cannot only exert potent antileishmanial activity but may also enhance cellular immunity to this parasite. Further studies are required to determine the main compound(s) responsible for these effects of the plant.
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نویسندگان مقاله رضا رضایی | reza rezaei
dept. of parasitology amp;amp; mycology, school of medicine, urmia university of medical sciences, urmia, iran cellular and molecular research center, urmia university of medical sciences, urmia, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ارومیه (Urmia university of medical sciences)

خسرو حضرتی تپه | khosrow hazrati tappeh
dept. of immunology, school of medicine, urmia university of medical sciences, urmia, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ارومیه (Urmia university of medical sciences)

شهرام سیدی | shahram seyyedi
dept. of pharmacology, school of medicine, urmia university of medical sciences, urmia, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ارومیه (Urmia university of medical sciences)

پیمان میکاییلی | peyman mikaili


نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1771
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-429505.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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