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International Journal of Hematology-Oncology and Stem Cell Research، جلد ۱۱، شماره ۳، صفحات ۱۷۲-۱۷۷
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عنوان انگلیسی Patterns of DNMT1 Promoter Methylation in Patients with Acute Lymphoblastic Leukemia
چکیده انگلیسی مقاله Background: Acute lymphoblastic leukemia (ALL) is a clonal malignant disorder characterized by an uncontrolled proliferation of immature T or B lymphocytes. Extensive studies have shown that the epigenetic changes, especially modified DNA methylation patterns in the regulatory regions through the DNA methyltransferase (DNMTs), play an important role in the development of genetic disorders and abnormal growth and maturation capacity of leukemic stem cells (LSCs).The aim of this study was to evaluate the changes in DNMT1 promoter methylation and its expression pattern in patients with ALL. Subjects and Methods: In this experimental study, methylation specific PCR (MSP) was used to assess the methylation status of DNMT1 promoter regions in samples collected from ALL patients (n=45) and healthy control subjects. According to this method, un-methylated cytosine nucleotides are converted to uracil by sodium bisulfite and the proliferation of methylated and un-methylated regions are performed using specific primers for target sequences. Results: None of the patients with B and T-ALL showed methylated promoter regions of the DNMT1 gene, while the methylation pattern of both pre-B ALL patients and the control group showed a relative promoter methylation. Conclusion: Analysis of promoter methylation patterns in various subgroups of ALL has revealed the importance of DNMT1 in the regulation of gene expression. Likewise, extensive data have also highlighted the methylation-based mechanisms exerted by DNAM1 as one of the main participants regulating gene expression in B-ALL and T-ALL patients. Investigation of the overall DNA methylation pattern offers significant improvements in the prediction of disease prognosis and treatment response.
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نویسندگان مقاله تیرداد رحمانی | tirdad rahmani
department of medical laboratory sciences, faculty of allied medicine, qazvin university of medical sciences, qazvin, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی قزوین (Qazvin university of medical sciences)

مهدی آزاد | mehdi azad
hematology-oncology and stemcell transplantation research center, tehran universityof medical science, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

بهرام چهاردولی | bahram chahardouli
hematology-oncology and stem cell transplantation research center, tehran university of medical science, tehran, iran children’s medical center, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

هاجر نصیری | hajar nasiri
department of hematology, faculty of allied medicine, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

موسی vatanmakanian | mousa vatanmakanian
department of hematology, school of medical sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

سعید کاویانی | saeid kaviani


نشانی اینترنتی http://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/675
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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