| چکیده انگلیسی مقاله |
Objective(s): The purpose of this study was to investigate the role of chloride channel protein 2 (ClC-2) in glutamate-induced apoptosis in the retinal ganglion cell line (RGC-5). Materials and Methods: RGC-5 cells were treated with 1 mM glutamate for 24 hr. The expression of ClC-2, Bax, and Bcl-2 was detected by western blot analysis. Cell survival and apoptosis were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry assays, respectively. Caspase-3 and -9 activities were determined by a colorimetric assay. The roles of ClC-2 in glutamate-induced apoptosis were examined by using ClC-2 complementary deoxyribonucleic acid (cDNA) and small inference ribonucleic acid (RNA) transfection technology. Results: Overexpression of ClC-2 in RGC-5 cells significantly decreased glutamate-induced apoptosis and increased cell viability, whereas silencing of ClC-2 with short hairpin (sh) RNA produced opposite effects. ClC-2 overexpression increased the expression of Bcl-2, decreased the expression of Bax, and decreased caspase-3 and -9 activation in RGC-5 cells treated with glutamate, but silencing of ClC-2 produced opposite effects. Conclusion: Our data suggest that ClC-2 chloride channels might play a protective role in glutamate-induced apoptosis in retinal ganglion cells via the mitochondria-dependent apoptosis pathway. |
| نویسندگان مقاله |
miao miao bi | miao miao bi
department of ophthalmology, the second hospital of jilin university, jilin university, changchun, jilin, 130041, p.r. china|department of ophthalmology, the china-japan :union: hospitial of jilin university, jilin university, changchun, jilin, 130033, xiantai street no. 126, jilin province, china
sen hong | sen hong
department of colon and anal surgery, the first hospital of jilin university, jilin university, changchun, jilin, 130021, p.r. china
ling جون ma | ling jun ma
department of ophthalmology, the china-japan :union: hospitial of jilin university, jilin university, changchun, jilin, 130033, xiantai street no. 126, jilin province, china
hong یان zhou | hong yan zhou
department of ophthalmology, the china-japan :union: hospitial of jilin university, jilin university, changchun, jilin, 130033, xiantai street no. 126, jilin province, china
جیا lu | jia lu
department of ophthalmology, the china-japan :union: hospitial of jilin university, jilin university, changchun, jilin, 130033, xiantai street no. 126, jilin province, china
جینگ zhao | jing zhao
department of ophthalmology, the china-japan :union: hospitial of jilin university, jilin university, changchun, jilin, 130033, xiantai street no. 126, jilin province, china
ya خوان zheng | ya juan zheng
department of ophthalmology, the second hospital of jilin university, jilin university, changchun, jilin, 130041, p.r. china
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