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Iranian Journal of Microbiology، جلد ۵، شماره ۴، صفحات ۳۷۴-۳۷۸
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عنوان انگلیسی Cloning, expression and purification of Mycobacterium tuberculosis ESAT-6 and CFP-10 antigens.
چکیده انگلیسی مقاله Background and Objectives: ESAT-6 (6-kDaearly secretory antigenic target) and CFP-10 (10-kDa culture filtrate protein) have been described as dominant antigens recognized by T-cells and considered as virulence factors in Mycobacterium tuberculosis . The aim of this study was to clone, express and purify recombinant ESAT-6 andCFP-10 proteins of M. tuberculosis in soluble form. Materials and Methods: ESAT-6 andCFP-10 genes were amplified by PCR, cloned into pET32a (+) vector, and overexpress- ed using isopropyl-beta-D-thiogalactopyranoside in E. coli BL21 (DE3). ESAT-6 andCFP-10 proteins were purified by Ni- NTA affinity chromatography and were detected by anti- ESAT-6 and anti -CFP10 antibodies. Results: ESAT-6 andCFP-10 genes were successfully expressed and purified. Anti- ESAT-6 and anti-CFP-10 antibodies were produced after induction of immunization against purified ESAT-6 andCFP-10 proteins in rabbit. Conclusion: In this study, we cloned, expressed and purified sufficient amounts of ESAT-6 andCFP-10 and it would be tested for the development of diagnostic kit for M. tuberculosis in future.
کلیدواژه‌های انگلیسی مقاله CFP-10,ESAT-6,M. tuberculosis
نویسندگان مقاله شیما محمودی | shima mahmoudi
pediatric infectious disease research center, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

ستاره ممیشی | setareh mamishi
pediatric infectious disease research center, tehran university of medical sciences, tehran, iran and department of infectious disease, children medical center hospital, school of medicine, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

مونا قاضی | mona ghazi
department of microbiology, school of medicine, shahid beheshti university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

ریحانه حسین پور صادقی | reihaneh hosseinpour sadeghi
pediatric infectious disease research center, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

بابک پوراکبری | babak pourakbari
pediatric infectious disease research center, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/501
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