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Iranian Journal of Basic Medical Sciences، جلد ۲۰، شماره ۱۰، صفحات ۱۱۰۲-۱۱۰۸
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عنوان انگلیسی The effects of anti-Fas ribozyme on T lymphocyte apoptosis in mice model with chronic obstructive pulmonary disease
چکیده انگلیسی مقاله Objective(s): In this study, we aimed to investigate the effects of anti-Fas ribozyme on the apoptosis of T lymphocytes (T cells) in mice model with chronic obstructive pulmonary disease (COPD). Materials and Methods: Male 6-week-old C57BL/6 mice were used to establish the COPD model by exposure to cigarette smoke. The COPD mice were sacrificed for spleen dissection and T cell isolation. T cells were randomly divided into four groups (n=10 per group). Group A was used as the control. B, C, and D groups were transfected with empty lentivirus, anti-Fas ribozyme, and an anti-Fas ribozyme mutant, respectively. The expression of Fas mRNA and protein in the T cells were evaluated using qPCR and Western blot, respectively. Flow cytometry was used to evaluate the apoptosis of CD4＋T cells and calculate the ratio of CD4+ to CD8+ T cells (CD4＋/CD8＋). Results: Anti-Fas ribozyme significantly inhibited the expression of Fas in the T cells of COPD mice. In addition, the number of apoptotic CD4＋ T cells and CD4＋/CD8＋of the C and D groups were significantly lower and higher than those of group A, respectively (P< 0.05). The apoptotic CD4＋T cells and CD4＋CD8＋of the C group were significantly lower and higher than those of group D, respectively (P< 0.05). Conclusion: Anti-Fas ribozyme significantly inhibited the expression of Fas, increased CD4+/ CD8+, and inhibited the apoptosis of T cells in COPD mice.
کلیدواژه‌های انگلیسی مقاله Apoptosis, Anti-Fas ribozyme, COPD mice model, Fas, T cells
نویسندگان مقاله song ming zhuo | song ming zhuo
department of respiratory medicine, the affiliated shenzhen longgang center hospital, zunyi medical university, shenzhen city, guangdong province, china


si cong li | si cong li
zhuhai campus of zunyi medical university, zhuhai city, guangdong province, china


یونگ qun lin | yong qun lin
department of respiratory medicine, the affiliated shenzhen longgang center hospital, zunyi medical university, shenzhen city, guangdong province, china


hai بین yu | hai bin yu
department of respiratory medicine, the affiliated shenzhen longgang center hospital, zunyi medical university, shenzhen city, guangdong province, china


| mojtaba fattahi abdizadeh
department of microbiology, faculty of medicine, sabzevar university of medical sciences, sabzevar, iran|department of virology, ahvaz jundishapur university of medical sciences, ahvaz, iran|virology department, pasteur institute of iran, 69 pasteur ave 1316943551. tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (Ahvaz jundishapur university of medical sciences)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_9367.html
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