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Iranian Journal of Basic Medical Sciences، جلد ۲۰، شماره ۱۰، صفحات ۱۱۰۹-۱۱۱۸
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عنوان انگلیسی A novel medium-throughput biological assay system for HTLV-1 infectivity and drug discovery
چکیده انگلیسی مقاله Objective(s): Here, a reporter cell line containing two reporter vectors were developed, in order to monitor the Human T-Lymphotropic Virus type1(HTLV-1) infectivity and the cell viability simultaneously. Materials and Methods: The reporter cell line was constructed by stably transfected baby hamster's kidney cell line (BHK-21), with the genomes expressing two different reporters in separate plasmids.The first reporter gene is transactivated by the HTLV-1 tax protein, while the second reporter is continuously expressed when introduced into a mammalian cell. In order to show its functionality, the effect of the drug mix on HTLV-1 was assayed by this system and was compared to the results obtained by other methods. Results: HTLV-1 reporter cell line was found to produce high level of luciferase when co-cultured with MT-2 and Hut-102 cells but not with Jurkat cell. Moreover, the combination therapy against HTLV-1 can reduce luciferase expression of the cell when co-cultured with MT-2 and Hut-102 comparable to the ELISA (R=0.932, P-value =0.002). In addition, the results revealed the superiority of the present system over the molecular methods. Conclusion: The results demonstrated that the biological assay system is a beneficial tool for the medium-throughput anti-HTLV-1 drug screening and inhibitory effect.
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نویسندگان مقاله | mojtaba fattahi abdizadeh
department of microbiology, faculty of medicine, sabzevar university of medical sciences, sabzevar, iran|department of virology, ahvaz jundishapur university of medical sciences, ahvaz, iran|virology department, pasteur institute of iran, 69 pasteur ave 1316943551. tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (Ahvaz jundishapur university of medical sciences)

منوچهر مکوندی | manoochehr makvandi
infectious and tropical diseases research center, ahvaz jundishapur university of medical sciences, ahvaz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (Ahvaz jundishapur university of medical sciences)

علیرضا سمرباف زاده | alireza samarbafzadeh
department of virology, ahvaz jundishapur university of medical sciences, ahvaz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (Ahvaz jundishapur university of medical sciences)

کیهان آزادمنش | kayhan azadmanesh
virology department, pasteur institute of iran, 69 pasteur ave 1316943551. tehran, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_9417.html
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