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Journal of Agricultural Science and Technology، جلد ۲، شماره ۳، صفحات ۱۶۷-۱۷۵
عنوان فارسی
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عنوان انگلیسی An Acetocarmine Staining Procedure for Chromosome Banding Studies of Immature Pollen in Triticeae
چکیده انگلیسی مقاله Studies of postmeiotic chromosomes have been impeded by the thick exine of immature pollen grains. Chromosome staining of immature and adult pollen grains through conven-tional acetocarmine procedure is tedious and often leads to unsatisfactory results. An acetocarmine stain, used in conjugation with Carnoy’s solution as fixative for 72 hrs or until the chlorophyll-containing tissues of spike became colorless, were successfully used to stain chromosome bands and nucleoli of the immature pollen grain in Aegilops cylin-drica. Mitosis of immature pollen exhibited several remarkable features, one being the distinguishable haploid chromosome set of immature pollen. Only one of two nucleolus organizer regions of Ae. cylindrica produced nucleolus at the prophase of first pollen mi-tosis. An acetocarmine-banded karyotype with only minor variations in the acetocarmine-banding pattern was revealed within and between plants of the accession of Ae. cylindrica. Some of banding patterns in the chromosome complement was identical to marker C-bands or accession-specific bands in corresponding genome in Ae. caudata (CC) or Ae. squarrosa (DD). The reliability of the acetocarmine banding for Triticeae chromosome identification is discussed.
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نویسندگان مقاله a ارزانی |
department of agronomy and plant breeding, college of agriculture, isfahan university of technology, isfahan-84156, islamic republic of iran.
سازمان اصلی تایید شده: دانشگاه صنعتی اصفهان (Isfahan university of technology)

m پورسیاه بیدی |
department of agronomy and plant breeding, college of agriculture, isfahan university of technology, isfahan-84156, islamic republic of iran.
سازمان اصلی تایید شده: دانشگاه صنعتی اصفهان (Isfahan university of technology)

e مرتضوی |
department of agronomy and plant breeding, college of agriculture, isfahan university of technology, isfahan-84156, islamic republic of iran.
سازمان اصلی تایید شده: دانشگاه صنعتی اصفهان (Isfahan university of technology)

نشانی اینترنتی http://jast.modares.ac.ir/article_4604_fba445e53a2a75da9b82c8e1f31ae310.pdf
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/476/article-476-495175.pdf
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زبان مقاله منتشر شده en
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