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Medical Journal of Islamic Republic of Iran، جلد ۲۶، شماره ۲، صفحات ۴۵-۴۹
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عنوان انگلیسی Comparison of cell wall proteins in putative Candida albicans & Candida dubliniensis by using modified staining method & SDSPAGE
چکیده انگلیسی مقاله  Background: Candida species are among the most common causes of opportunistic fungal diseases. Among Candida species, Candida albicans is responsible for most infections. Having many strains, C. albicans is very polymorph. C. dubliniensis is very similar to albicans species both morphologically and physiologically. For an infection to occur, cell wall proteins play an important role as they enable yeast to adhere to host cells and begin pathogenesis. Therefore, we decided to extract these proteins and examine them through common molecular methods of protein analysis including SDS-PAGE. Methods: Initially cell wall proteins of two C. albicans strains (CBS 562 and PTCC6027) and one C. dubliniensis strain (CBS7987) were extracted by using a solution of beta-mercaptoethanol and ammonium carbonate. After dialysis against Tris-HCL buffer, SDS gel electrophoresis was performed on the proteins extract. Bands were then visualized by using three different staining methods among which one method provided improved detection. Results: By using Coomassie Brilliant Blue staining method, proteins with molecular weight of 42, 66.2 and 200 kDa were detected. By using Silver staining method, proteins with molecular weight of 21.5, 28.5 and 37 kDa were detected. However, using combined Coomassie Brilliant Blue & Sliver staining method visualized more bands resulting in improved detection. Conclusion: To answer many existing questions about fungal diseases, fungi cell wall proteins are necessary to be examined. To commence such examinations, a simple step may be an SDS-PAGE performance on as many strains as possible. A combined staining method can enhance bands detection.
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نویسندگان مقاله سید امیر یزدان پرست | seyed amir yazdanparast
department of medical mycology, school of allied health sciences,tehran university of medical sciences, shahid hemmat expressway, shahid chamran crossway, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

سید شهرزاد مهدوی نظارتی | seyedeh shahrzad mahdavi nezarati
department of microbiology, islamic azad university, tehran north branch

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

فریبا حشمتی | fariba heshmati
department of medical microbiology, school of allied health sciences, tehran university of medical sciences,

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

سپیده حمزه لو | sepideh hamzehlou
department of medical biotechnology, school of allied health sciences, tehran university of medical sciences

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-297&slc_lang=en&sid=en
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زبان مقاله منتشر شده en
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