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Iranian Journal of Parasitology، جلد ۱۲، شماره ۴، صفحات ۵۲۲-۵۲۳
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عنوان انگلیسی Phylogenetic Analysis of Giardia lamblia Human Genotypes in Fars Province, Southern Iran
چکیده انگلیسی مقاله Abstract Background: This study is the first phylogenetic genotype analysis of Giardia lamblia in Iran. The main objective was to determine genotyping and identify the sub-assemblages of Giardia lamblia isolates involved in the transmission of giardiasis in Fars Province, south of Iran, in 2012. Methods: Forty G. lamblia isolates were collected from the patient’s fecal samples with gastrointestinal discomfort referred to the health centers and hospitals in Shiraz, Fars Province, south of Iran. Purification of G. lamblia cysts from fecal samples and DNA extraction were performed using monolayer of sucrose density gradient and Phenol-Chloroform-Isoamylalcohol (PCI) respectively. Semi-nested PCR and sequence analysis were then performed using the primers (GDHeF, GDHiF, and GDHiR) which amplified a 432-bp fragment of Giardia glutamate dehydrogenase ( gdh ) gene. Phylogenetic analysis was carried out using a neighbor-joining tree composed of the nucleotide sequences of G. lamblia isolates obtained in this study and the known sequences isolates published in GenBank. Results: G. lamblia sub-assemblage AII was the most prevalent genotype with 80% of the cases and 20% of the cases belong to sub-assemblage BIII and BIV based on the DNA sequence of the gdh . G. lamblia isolates at Fars Province were widely distributed within assemblage A cluster (sub-assemblage AII) and the remaining isolates were dispersed throughout the assemblage B cluster (sub-assemblage BIII and BIV). Conclusion: PCR Sequencing and phylogenetic analysis was a proper molecular method for genotyping and discriminating of the of G. lamblia sub-assemblages in fecal samples, using the glutamate dehydrogenase gene that suggests a human contamination origin of giardiasis.
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نویسندگان مقاله محمد ریانی | mohammad rayani
basic sciences in infectious diseases research center, school of advanced medical sciences and technologies, shiraz university of medical sciences, shiraz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

غلامرضا حاتم | gholamreza hatam
dept. of medical microbiology and parasitology, faculty of medicine and health sciences, university putra malaysia, serdang, malaysia


ngah zasmy unyah | ngah zasmy unyah
basic sciences in infectious diseases research center, school of advanced medical sciences and technologies, shiraz university of medical sciences, shiraz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

عبدالمجید ashrafmansori | abdolmajid ashrafmansori
dept. of medical microbiology and parasitology, faculty of medicine and health sciences, university putra malaysia, serdang, malaysia


wan عمر عبدالله | wan omar abdullah
dept. of medical microbiology and parasitology, faculty of medicine and health sciences, university putra malaysia, serdang, malaysia


rukman آونگ hamat | rukman awang hamat


نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1926
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زبان مقاله منتشر شده en
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