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Iranian Journal of Parasitology، جلد ۱۲، شماره ۴، صفحات ۵۵۴-۵۶۲
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عنوان انگلیسی Prevalence of Toxoplasma gondii Infection among Healthy Blood Donors in Northeast of Iran
چکیده انگلیسی مقاله Abstract Background: This cross-sectional investigation aimed to evaluate the prevalence of IgM and IgG anti- Toxoplasma gondii antibodies and the associated risk factors among healthy blood donors in Khorasan Razavi Province, northeast of Iran from Nov 2014 to May 2015. Methods: Overall, 491 serum samples from apparently healthy blood donors referred the six biggest blood centers in Razavi Khorasan, Iran, were screened for IgG and IgM anti- T. gondii antibodies by enzyme-linked immunosorbent assay (ELISA). A structured questionnaire was used to obtain information on risk factors for T. gondii infection. Nested PCR was also used to detect DNA of T. gondii in the IgM-positive samples by using of B1 and RE (Repetitive Element) as marker for amplifying fragment size of 531 bp and 164 bp in PCR method. Results: Totally, 200 (40.7%) samples were seropositive for anti- T. gondii antibodies; 184 (37.5%) donors tested seropositive for only IgG antibody, 8 (1.6%) tested seropositive for both IgM and IgG and 8 (1.6%) were positive for IgM antibody alone. Several risk factors significantly related to T. gondii seropositivity in the univariate analysis at P < 0.05 included age ( P < 0.001), and raw/half-cocked meat consumption ( P =0.015). T. gondii DNA was found in all sixteen IgM-positive samples. Conclusion: T. gondii infection was present among healthy blood donors in northeast of Iran. Thus, it is suggested to design screening programs for preventing transfusion-transmitted toxoplasmosis.
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نویسندگان مقاله سعید زرین گل | saeed sadooghian
dept. of medical parasitology and mycology, kerman university of medical sciences, kerman, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

حسین محمودوند | hossein mahmoudvand
blood transfusion organization of khorasan razavi, mashhad, iran

سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)

محمد علی محمدی | mohammad ali mohammadi


ناصر نظری سرچشمه | naser nazari sarcheshmeh
dept. of medical parasitology and mycology, kerman university of medical sciences, kerman, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

امیر توکلی kareshk | amir tavakoli kareshk
dept. of medical parasitology and mycology, kerman university of medical sciences, kerman, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

حسین کامیابی | hossein kamiabi
dept. of medical parasitology and mycology, kerman university of medical sciences, kerman, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

ناصر ضیا علی | naser zia ali


نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1929
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زبان مقاله منتشر شده en
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