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Medical Journal of Islamic Republic of Iran، جلد ۳۱، شماره ۱، صفحات ۳۶۸-۳۷۶
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی Antibacterial activity of amino- and amido- terminated poly (amidoamine)-G6 dendrimer on isolated bacteria from clinical specimens and standard strains
چکیده انگلیسی مقاله     Background: Nanoscale poly (amidoamine) dendrimers have been investigated for their biological demands, but their antibacterial activity has not been widely discovered. Thus, the sixth generation of poly (amidoamine) dendrimer (PAMAM-G6) was synthesized and its antibacterial activities were evaluated on Gram-negative bacteria; P. aeruginosa, E. coli, A. baumannii, S. typhimurium, S. dysenteriae, K. pneumoniae, P. mirabilis, and Gram-positive bacteria, and S.aureus and B. subtilis, which were isolated from different clinical specimens and standard strains of these bacteria.    Methods: In this study, 980 specimens including urine (47%), blood (27%), sputum (13%), wounds (8%), and burns (5%) were collected from clinical specimens of 16 hospitals and clinics in city of Sabzevar, Iran. Then, the target bacteria were isolated and identified using standard methods. Minimum inhibitory concentration and minimum bactericidal concentrations against Gram-positive and Gram-negative bacteria were determined according to guidelines described by clinical and laboratory standards institute (CLSI). Standard discs were prepared using 0.025, 0.25, 2.5, and 25 μg/mL concentrations of PAMAM-G6 on Mueller-Hinton agar plates to determinate the zone of inhibition. The cytotoxicity of PAMAM-G6 dendrimer was evaluated in HCT116 cells by MTT assay.    Results: The most important isolated bacteria were E. coli (23.65%), S. aureus (24.7%), P. aeruginosa (10.49%), B. subtilis (7.7%), S. typhimurium (8.87%), A. baumannii (7.02%), K. pneumoniae (7.1%), P. mirabilis (6.46%), and S. dysenteriae (3.6%). Moreover, it was found that poly (amidoamine)–G6 exhibited more antibacterial efficacy on standard strains than isolated bacteria from clinical samples (p< 0.05). The cytotoxicity of PAMAM-G6 to the cells showed that cytotoxicity depended on the concentration level and exposure time.    Conclusion: The PAMAM-G6 dendrimer showed a positive impact on the removal of dominant bacterial isolated from clinical specimens and standard strains.   
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نویسندگان مقاله ایوب رستگار | ayoob rastegar
department of environmental health engineering, school of public health, sabzevar university of medical sciences, sabzevar, iran amp; student research committee, faculty of public health branch, iran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

شهرام نظری | shahram nazari
student research committee, faculty of public health branch, iran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

احمد اله آبادی | ahmad allahabadi
department of environmental health engineering, school of public health, sabzevar university of medical sciences, sabzevar, iran.


فرحناز falanji | farahnaz falanji
cellular and molecular research center, sabzevar university of medical sciences, sabzevar, iran.


فخرالدین اکبری دورباش | fakhreddin akbari dourbash
department of materials science and engineering, tarbiat modares university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

زهرا رضایی | zahra rezai
department of environmental health engineering, school of public health, sabzevar university of medical sciences, sabzevar, iran.


سودابه علیزاده متبوع | soudabeh alizadeh matboo
school of public health, ardabil university of medical sciences, ardabil, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اردبیل (Ardabil university of medical sciences)

رضا حکمت شعار | reza hekmat shoar
department of occupational health engineering, school of public health, sabzevar university of medical sciences, sabzevar, iran.


سید محسن محسنی | seyed mohsen mohseni
department of environmental health engineering, school of public health, shahid beheshti university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

غریب مجیدی | gharib majidi
research center for environmental pollutants, qom university of medical sciences, qom, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی قم (Qom university of medical sciences)

نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-2906-1&slc_lang=en&sid=en
فایل مقاله دریافت فایل مقاله
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Microbiology and Anatomy
نوع مقاله منتشر شده Original Research
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