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Research in Molecular Medicine، جلد ۵، شماره ۲، صفحات ۲۸-۳۳
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی Bioinformatics Designing of 10-23 Deoxyribozyme against Coding Region of Beta-galactosidase Gene
چکیده انگلیسی مقاله Background: Deoxyribozymes (Dzs) can play a role as gene expression inhibitors at mRNA level. Among Dzs, the 10-23 deoxyribozyme has significant potentials for treatment of diseases. Designed Dz includes a catalytic core made of 15 deoxyribonucleotides and two binding arms consisted of 6-12 nucleotides for site specific binding to target RNA and hydrolysis. The enzyme has characteristic features for cleavage of the RNA target between an unpaired purine (A, G) and a paired pyrimidine (C or U). In this study, 10-23 Dz is designed for the coding region of the α-peptide of a lacZ gene. Material and Methods: The primary sequence of a plasmid with α-complementation ability was taken from addgene database. To confirm sequence validity, ExPASy was used to analyze related ORFs for the retrieved sequence. The ORF with identical sequence to α-peptide was selected in the reverse complement sequence. Subsequently, the secondary structure of the α-peptide was analyzed in DINAMelt web server and Mfold software. Then the intended target site was selected inside the coding region of the α-peptide. The Dzs sequence was designed for the target site with nucleotide binding arms. Results and conclusion: The resulted Dz in this study can be used as a promising catalytic DNA inside bacterial cells for blue-white screening. Criteria such as biological stability and catalytic rate of such enzymes must be evaluated in vivo and in vitro.
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نویسندگان مقاله نسرین al سادات احمدی | nasrin al sadat ahmadi
cellular and molecular biology division; department of biology; faculty of sciences, university of isfahan; isfahan; iran

سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)

ابوالقاسم اسماعیلی | abolghasem esmaeili
cellular and molecular biology division; department of biology; faculty of sciences, university of isfahan; isfahan; iran

سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)

فاطمه جوادی زرنقی | fatemeh javadi zarnaghi
cellular and molecular biology division; department of biology; faculty of sciences, university of isfahan; isfahan; iran

سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)

نشانی اینترنتی http://rmm.mazums.ac.ir/browse.php?a_code=A-10-751-2&slc_lang=en&sid=en
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده پژوهشی
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