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Medical Journal of Islamic Republic of Iran، جلد ۳۱، شماره ۱، صفحات ۶۹۳-۶۹۸
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عنوان انگلیسی Cloning, expression, and spectral analysis of mouse betatrophin
چکیده انگلیسی مقاله Background: Betatrophin, a novel secretory protein from liver and fatty tissues, is believed to be involved in lipid and glucose metabolism. However, its precise physiological role remains unclear. Here, we report the cloning, expression, and purification steps of mouse betatrophin in a prokaryotic system, followed by its structural analysis.    Methods: Specific cloning primers were used to amplify the coding sequence of mouse liver betatrophin. The product was cloned into pET28 and expressed in E.coli BL21 (DE3) cells. The suitability of the refolding procedure was assessed by determining secondary structures of the initial and refolded proteins using circular dichroism spectroscopy.    Results: The polymerase chain reaction resulted in a 549 bp nucleotide sequence, encoding a 183 amino acid polypeptide, with an apparent molecular weight of 21 kDa, which was expressed in an inclusion body. Following an optimization and refolding procedure, the recombinant protein was purified by anion exchange and metal affinity chromatography. CD spectra revealed that the refolded protein has suitable configuration.    Conclusion: We believe that the produced betatrophin is suitable for further biochemical studies on glucose and lipid metabolism.    
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نویسندگان مقاله سمانه غلامی | samaneh gholami
immunology research center, iran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

کوروش falak r iums ac ir | koroush goodarzvand chegini
department of biotechnology, qazvin university of medical sciences, qazvin, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی قزوین (Qazvin university of medical sciences)

نعمت اله غیبی | nematollah gheibi
department of biotechnology, qazvin university of medical sciences, qazvin, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی قزوین (Qazvin university of medical sciences)

کبری مختاریان | kobra mokhtarian
immunology research center, iran university of medical sciences, tehran, iran amp; medical plant research center, shahrekord university of medical sciences, shahrekord, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

محسن محمدی | mohsen mohamadi
immunology research center, iran university of medical sciences, tehran, iran amp; the persian gulf marine biotechnology medicine research center, bushehr universi-ty of medical sciences, bushehr, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

رضا فلک | reza falak
immunology research center, iran university of medical sciences, tehran, iran amp; department of immunology, school of medicine, iran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-2201-1&slc_lang=en&sid=en
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/18/article-18-567965.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Biotechnology
نوع مقاله منتشر شده Original Research
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