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Medical Journal of Islamic Republic of Iran، جلد ۳۱، شماره ۱، صفحات ۵۷۲-۵۷۸
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عنوان انگلیسی Evaluation of age effects on doxorubicin-induced toxicity in mesenchymal stem cells
چکیده انگلیسی مقاله Background: Doxorubicin, by aggregating in bone marrow, causes genotoxic effects, and thus reduces the repair ability of cells. The present study was conducted as an in vitro evaluation of age effects on the cytotoxicity induced by doxorubicin in mesenchymal stem cells (MSCs).    Methods: The MSCs of female BALB/c mice aged 1, 8, and 16 months were separated, characterized, and subsequently evaluated in cellular growth media. After 24 hours, exposure of the MSCs of the 3 groups of mice to doxorubicin (25, 50, 100, 200, 400, 800, 1200 nM) and cytotoxicity were assessed, and the sublethal dose was determined using flow cytometry technique and lactate dehydrogenase (LDH) release assay.    Results: The IC50 values determined by flow cytometry for the separated MSCs of 1 young, 8 middle- aged, and 16 old mice were and respectively. Interestingly, the results of these 2 methods in determining cytotoxicity were in agreement, and a concentration of approximately 25 nM was considered to be the shared sublethal dose for different ages.    Conclusion: The results indicated that MSCs of middle-aged mice were more resistant to the toxic effects of the drug. Besides, MSCs separated from the old mice were the most sensitive to chemotherapy and its side effects such as disruptions of cell proliferation and viability. These disruptions can be ascribed to the alteration of function and physiological processes with age. Determining proper concentration of doxorubicin drug to destruct cancerous cells based on age and individual sensitivity can minimize the amount of toxicity.  
کلیدواژه‌های انگلیسی مقاله Doxorubicin, Mesenchymal Stem Cells, Cytotoxicity, Age
نویسندگان مقاله علی بشیری دزفولی | ali bashiri dezfouli
department of basic sciences, faculty of veterinary medicine, university of tehran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

علی اکبر پورفتح اله | ali akbar pourfathollah
department of immunology, faculty of medical science, tarbiat modares university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

جمیله سالار آملی | jamileh salar amoli
department of basic sciences, toxicology and animal poisoning research center, faculty of veterinary medicine, university of tehran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

محمد خسروی | mohammad khosravi
department of pathobiology, faculty of veterinary medicine, shahid chamran univer-sity of ahvaz, ahvaz, iran.


مهین نیکوگفتار ظریف | mahin nikogoftar zarif
blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran.

سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)

مینا یزدی | mina yazdi
faculty of veterinary medicine, university of tehran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

طاهره علی اصفهانی | tahereh ali esfahani
toxicology and animal poisoning research center, faculty of veterinary medicine, university of tehran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-3412-2&slc_lang=fa&sid=en
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زبان مقاله منتشر شده en
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