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Research in Pharmaceutical Sciences، جلد ۱۱، شماره ۳، صفحات ۱۷۷-۰
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عنوان انگلیسی Inhibition of P-glycoprotein expression and function by anti-diabetic drugs gliclazide, metformin, and pioglitazone in vitro and in situ
چکیده انگلیسی مقاله P-glycoprotein (P-gp) is a trans-membrane drug efflux pump. Several drugs are P-gp substrates. Some drugs may affect the activity of P-gp by inhibiting its function, resulting in significant drug-drug interactions (DDIs). It is critical to understand which drugs are inhibitors of P-gp so that adverse DDIs can be minimized or avoided. This study investigated the effects of gliclazide, metformin, and pioglitazone on the function and expression of P-gp. Rhodamine 123 (Rh 123) efflux assays in Caco-2 cells and western blot testing were used to study in vitro the effect of the drugs on P-gp function and expression. The in situ rat single-pass intestinal permeability model was developed to study the effect of the drugs on P-gp function. Digoxin and verapamil were used as a known substrate and inhibitor of P-gp, respectively. Digoxin levels in intestinal perfusion samples were analyzed by high-performance liquid chromatography. Intestinal effective permeability (P eff ) of digoxin in the presence of 0.1, 10, and 500 µM gliclazide, 100 and 7000 µM metformin, and 50 and 300 µM pioglitazone was significantly increased relative to the digoxin treated cells ( P < 0.01). P-gp expression was decreased by gliclazide, metformin and pioglitazone. Intracellular accumulation of Rh 123 by the drugs increased, but the differences were not significant relative to the control cells ( P > 0.05). It was found that gliclazide, metformin, and pioglitazone inhibited P-gp efflux activity in situ and down-regulated P-gp expression in vitro . Further investigations are necessary to confirm the obtained results and to define the mechanism underlying P-gp inhibition by the drugs.
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نویسندگان مقاله مهران مسگری عباسی | mehran mesgari abbasi


هادی ولی زاده | hadi valizadeh


حامد همیشه کار | hamed hamishehkar


پروین ذاکری میلانی | parvin zakeri milani


نشانی اینترنتی http://rps.mui.ac.ir/index.php/jrps/article/view/1688
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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