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Journal of Agricultural Science and Technology، جلد ۹، شماره ۴، صفحات ۳۴۱-۳۴۷
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عنوان انگلیسی Serological Characterization of Alfalfa Mosaic Virus in Alfalfa (Medicago sativa) in Some Regions of Iran
چکیده انگلیسی مقاله A survey was carried out in five provinces of Iran (Kerman, Sistan and Baluchestan, Hormozgan, Khorasan and Yazd) for the presence of Alfalfa mosaic virus (AMV) sero-types in alfalfa during 2002 to 2003. The number of samples collected was 250, represent-ing the diversity and geographical distribution of AMV in these areas. Diagnosis was car-ried out using polyclonal (PAbs) and monoclonal (MAbs) antibodies. A total of 110 symp-tomatic leaf samples gave a positive reaction in ELISA with polyclonal antibodies. Twelve out of 20 MAbs reacted with all samples tested and were considered as non-differentiating MAbs. Only the MAbs-12, 13, 15, 21, 22 and 24 gave a clear differential reaction and were used for identifying AMV serotypes. Two MAbs (1 and 2) did not react with AMV posi-tive samples. Serological relatedness among AMV samples was studied by indicating the existence of six serotypes of AMV strains in the surveyed areas.
کلیدواژه‌های انگلیسی مقاله AMV,Monoclonal antibodies,Serotype,Strain
نویسندگان مقاله | H. Massumi
Department of Plant Pathology, Faculty of Agriculture, Shahid Bahonar University of Kerman, P. O. Box: 76169-133, Kerman, Islamic Republic of Iran.


| A. Hosseini Pour
Department of Plant Pathology, Faculty of Agriculture, Shahid Bahonar University of Kerman, P. O. Box: 76169-133, Kerman, Islamic Republic of Iran.
نشانی اینترنتی http://journals.modares.ac.ir/browse.php?a_code=A-23-1000-5917&slc_lang=en&sid=23
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