این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
International Journal of Hematology-Oncology and Stem Cell Research، جلد ۱۲، شماره ۳، صفحات ۲۰۳-۲۱۱
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Cytotoxic and apoptogenic activity of Bryonia aspera extract on pre-B acute lymphoblastic leukemia cell lines
چکیده انگلیسی مقاله Background: The natural products and conventional chemotherapeutic drug combinations are believed to increase cure rates of anticancer treatment while reducing its toxicity. The current study investigates cytotoxic and apoptogenic effects of methanolic extract of Beryonia aspera , and also synergistic effects of this extract and Prednisolone on acute lymphoblastic leukemia cell lines. Materials and Methods: The under study populations were NALM-6 and REH cell lines. Cells were treated by Prednisolone and B. aspera extract alone and in combination. The effect of the drugs on survival and apoptosis were examined using MTT and flow cytometry, respectively. Moreover, the effects of the drugs on the mRNA expression levels of Bax and Bcl-2 were studied using RQ-PCR. Finally, both the transcriptional and enzymatic activity of caspase-3 were investigated by caspase-3 assay kit. Results: The B. aspera extract induced cell growth inhibition and triggered apoptosis in a dose- and time-dependent manner. Real-time PCR analysis of apoptotic target genes revealed that this agent shifted the ratio of the death promoter to death repressor genes via alteration of Bax and Bcl-2 expression levels. These changes resulted in caspase-3 activation, which led to DNA fragmentation and subsequent apoptosis. Our study has also demonstrated that the combined treatment of B. aspera extract with Prednisolone did not induce greater cytotoxic effect as compared to treatment series using either Prednisolone alone. Conclusion: Our study demonstrated that the B. aspera extract has anti-leukemic properties on BCP-ALL cell lines and could be regarded as a promising agent for the treatment of ALL.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Sorur Yazdanpanah
Traditional Medicine and Materia Medica Research Center (TMRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Somayeh Esmaeili
Department of Laboratory Hematology and Blood bank, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Davood Bashash
Department of Laboratory Hematology and Blood bank, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Nasrin Dehghan Nayeri
Department of Laboratory Hematology and Blood bank, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Mohammad Esfini Farahani
Department of Laboratory Hematology and Blood bank, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.


| Ahmad Gharehbaghian


نشانی اینترنتی http://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/872
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/78/article-78-694635.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article(s)
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات