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Iranian Journal of Basic Medical Sciences، جلد ۲۱، شماره ۹، صفحات ۹۶۵-۹۷۱
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عنوان انگلیسی The osteogenesis of bacterial cellulose scaffold loaded with fisetin
چکیده انگلیسی مقاله Objective(s): Bacterial cellulose (BC) has applications in medical science, it is easily synthesized, economic and purer compared to plant cellulose. The present study aimed to evaluate BC, a biocompatible natural polymer, as a scaffold for the bone marrow mesenchymal stem cells (BMSCs) loaded with fisetin, a phytoestrogen.Materials and Methods: BC hydrogel scaffold was prepared from Gluconaceter xylinus and characterized through scanning electron microscopy (SEM). Biocompatibility of BC was measured by MTT assay, BMSCs were obtained from femur of rat and the osteogenic potential of the BC scaffold cultured with BMSCs and loaded with fisetin, was investigated by measuring the alkaline phosphatase (ALP) activity, alizarin red staining (ARS) and real-time PCR in terms of osteoblast-specific marker, osteocalcin (OCN) and osteopontin (OPN). Results: Biocompatibility results did not show any toxic effects of BC scaffold on BMSCs, while it increased cell viability. The data showed that BC loaded fisetin differentiated BMSCs into osteoblasts as demonstrated by ALP activity assays and ARS in vitro. Moreover, results from gene expression assay showed the expression of OCN and OPN genes was increased in cells that were seeded on the BC scaffold loaded with fisetin.Conclusion: According to the results of the present study, BC loaded with fisetin is an effective strategy to promote osteogenic differentiation and a proper localized delivery system, which could be a potential candidate in bone tissue engineering.
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نویسندگان مقاله | Elahe Vadaye kheiry
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran


| kazem Parivar
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran


| Javad Baharara
Research Center for Animal Development and Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran


| Bibi Sedigheh Fazly Bazzaz
Biotechnology Research Center, Institute of Pharmaceutical Technology, Mashhad University of Medical Sciences, Mashhad, Iran|School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran


| Alireza Iranbakhsh
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
نشانی اینترنتی http://ijbms.mums.ac.ir/article_11265.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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