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Medical Journal of Islamic Republic of Iran، جلد ۱۹، شماره ۲، صفحات ۱۸۱-۱۸۴
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عنوان انگلیسی DETECTION OF HAEMOPHILUS INFLUENZAE TYPE B IN CEREBROSPINAL FLUID OF SUSPECTED CHILDREN WITH MENINGITIS BYPCR
چکیده انگلیسی مقاله  ABSTRACT Background: A polymerase chain reaction (PCR) assay with primers from "hpd" gene was compared with culture for the detection of Haemophilus influenzae type b (Hib) in CSF samples from children suspected of meningitis. Methods: 300 cerebrospinal fluid (CSF) samples from suspected meningitis cases were studied by culture (Leventhal & Chocolate Agar) and PCR. Results: The latter method could detect Hib in all 5 culture positive and in 2 of 295 culture negative cases, showing sensitivity, specificity, and an accuracy index of 100%, 99% and 99%, respectively. The PCR result was available within a day. Conclusion: This study has shown that PCR is a rapid, sensitive, and specific diagnostic test for Hib from CSF samples. Futthermore, to maximize management of meningitis cases to reduce the morbidity, mortality, and complications of invasive Hib infection a combination of culture and PCR is necessary for the detection ofHib infection. The incidence ofHib meningitis among children in Children's Medical Center in Tehran based on culture and PCR results were 2.4% and 1.7% respectively. Based on culture results, Hib causes 15.6% of all cases ofbacte1ial meningitis in children. 
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نویسندگان مقاله فروغ اعظم نخجوانی | f a nakhjavani
departllle ll of v icrobiologv. facultr oj apos;medicine. tehran universin o,{medical sciences


f بنکدار هاشمی | f bonakdar hashemi
department of microbiology. faculty of medicine. tehran university of medical sciences

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محمدتقی کلانی | m t kalani
department of microbiology. faculty of medicine. tehran university of medical sciences

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

b کاظمی | b. kazemi
departmzent of apos;parasiology, faculty of apos; medicine. shahid beheshti universitv of medical sciences


k نوری | k. nouri
department of biostatistics and epidemiology. faculty of apos;public health amp; lnstiture of health research, tehran universitv of medical sciences.


n آزادی | n. azadi
department of biostatistics and epidemiology. faculty of apos;public health amp; lnstiture of health research, tehran universitv of medical sciences.


m حقی آشتیانی | m haghi ashtiani
children apos;s medical center; microbiology laboratory.tehran university of apos; medical sciences, tehran. i.r.iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

m علیقلی | m. aligholi
department of microbiology. faculty of medicine. tehran university of medical sciences

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

u عرفانی | u. erfani
children apos;s medical center; microbiology laboratory.tehran university of apos; medical sciences, tehran. i.r.iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

m عابدینی | m. abedini
children apos;s medical center; microbiology laboratory.tehran university of apos; medical sciences, tehran. i.r.iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-310&slc_lang=en&sid=en
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