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Medical Journal of Islamic Republic of Iran، جلد ۱۹، شماره ۱، صفحات ۵۳-۵۶
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عنوان انگلیسی ADENOSINE DEAMINASE ACTIVITY IN ESTROGEN RECEPTOR POSITIVE AND NEGATIVE HUMAN BREAST CANCER CELL LINES
چکیده انگلیسی مقاله  ABSTRACT Background: The aims of this study were to assay the activity of adenosine deaminase (ADA) in estrogen receptor positive (MCF-7) and negative (MDA-MB468) breast cancer cell lines. Methods: MDA-MB468 and MCF-7 breast cancer cell lines were cultured in complete medium, striped serum with and without 0.0 1~-LM diethylstilbestrol (DES), complete medium in the presence and absence of 111M tamoxifen for 20 hr. Adenosine deaminase activity was determined using the colorimetric method described by Guisti and Galanti. Results: It was found that the activity of enzyme in estrogen receptor positive (ER+) cell line (MCF-7) was significantly higher than that of estrogen receptor negative breast cancer cell line (MDA-MB468). ADA activity in MCF-7 cells cultured in the presence of tamoxifen or charcoal-striped serum was significantly lower than that of control. Furthermore addition of diethylstilbestrol (DES) to the striped serum increased the value of ADA activity to that of control. Unlike MCF-7 cells, the activity of ADA in MDA-MB468 cells remained unchanged upon treatment with tamoxifen or striped serum. Conclusion: These findings suggest estrogen responsiveness of ADA expression in MCF-7 cells.
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نویسندگان مقاله محمد هاشمی | mohammad hashemi
dept. of clinical biochemistry, zahedan medical university, zahedan , iran .


فاطمه کرمی تهرانی | fatemeh karami tehrani
cancer research lab, dept. of clinical biochemistry, school of medical sciences, tarbiat modarres university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

سعید قوامی | saeid ghavami
dept. of clinical biochemistry, school of medicine, zahedan medical university, zahedan, iran,


مجید سیرتی ثابت | majid sirati sabet
dept. of clinical biochemistry, school of medicine, qazvin medicaluniversity, qazvin, iran.
نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-261&slc_lang=en&sid=en
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده 1
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