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Medical Journal of Islamic Republic of Iran، جلد ۱۸، شماره ۳، صفحات ۲۵۷-۲۶۳
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عنوان انگلیسی EXPANSION OF HUMAN CORD BLOOD PRIMITIVE PROGENITORS IN SERUM-FREE MEDIA USING HUMAN BONE MARROW MESENCHYMAL STEM CELLS
چکیده انگلیسی مقاله Ex vivo expansion of human umbilical cord blood cells (HUCBC) is explored by several investigators to enhance the repopulating potential of HUCBC. The proliferation and expansion of human hematopoietic stem cells (HSC) in ex vivo culture was examined with the goal of generating a suitable clinical protocol for expanding HSC for patient transplantation. Using primary human mesenchymal stem cells, we established a serum-free culture system to expand human primitive progenitors and transplantable stem cells. Non-enriched cord blood CD34+ cells were cultured on a monolayer of human mesenchymal stem cells in the presence of tlu-ombopoietin (TPO), flt31flk2 ligand (FL), and/or stem cell factor (SCF), interleukin 6 (IL-6), interleukin 3 (IL-3) under serum-free conditions. After I or 2 weeks of culture, cells were examined for clonogenic progenitors and percentage of CD34+ CD38- cells. In the presence of TPO, FL, and SCF, fetal MSC cells supported more than a 35- and 20-fold expansion of CD34+ cells and colonyforming units in culture after 1 and 2 weeks of incubation, respectively. In addition, LTC-IC assay were expanded more than 7- and 16-fold after 1 and 2 weeks of culture, respectively. UCB-HSC can be expanded in culture to numbers theoretically adequate for safe, rapid engraftment of adult patients. Additional studies are needed to establish the functional activity of expanded UCB-HSC. This ex vivo expansion system should prove valuable in clinical settings in which stromal cells are available from recipients or stem cell donors.
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نویسندگان مقاله m solelmani | m solelmani
from the hematology dept .. school of apos;medical sciences. tarbiat modares university, tehran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

h مزدارانی | h mozdarani
medical genetics dept. school of medical sciences, tarbiat modares university, tehran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

aa پورفتح اله | aa pourfathollah
from the hematology dept .. school of apos;medical sciences. tarbiat modares university, tehran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

y مرتضوی | y mortazavi
from the hematology dept .. school of apos;medical sciences. tarbiat modares university, tehran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

k علی مقدم | k alimoghaddam
bone marrow transplantation center. shariati hospital, tehran,


a حاجی فتحعلی | a hajifathali
the iranian blood trans ilsion organization,tehran


z ذنوبی | z zonobi
shahid beheshti medical sciences university. tehran. iran
نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-60&slc_lang=en&sid=en
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