این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Medical Journal of Islamic Republic of Iran، جلد ۱۸، شماره ۱، صفحات ۵۵-۶۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی THE CYTOTOXICITY PATHWAY OF NATURAL KILLER CELLS IN CORD BLOOD COMPARED TO P ERIPHERAL BLOOD
چکیده انگلیسی مقاله The cytotoxic activity of natural killer cells is usually tested by radioactive assay CSI Cr release assay), which detects the release of cytoplasmic contents after plasma membrane disintegration of dying cells. In contrast to this indirect evaluation of cytotoxicity, the assessment of cell damage by flow cytometry aims to provide a more exact characterization of the death pathway via detection of the percentage of apoptosis and necrotic cells. Annexin V-FITC (Axv -FITC) can be used to label cells in the early apoptotic state, while propidiurn iodide (PI) indicates late apoptosis or necrosis. The NK cytotoxicity of cord blood (CB) and peripheral blood (PB) was determined after 4 hours of incubation in the absence of cytokines. After 4 hours in vitro incubation, co-staining with Annexin V-FITC (Axv-FITC) and propidiurn iodide (PI) pelmitted discrimination between viable, early apoptotic and necrotic cells. As we would expect, the cytotoxicity pathway in PB mononuclear cells (MNCs) consists of both apoptosis and necrosis pathways but in CB MNCs it almost consists of early apoptosis and necrosis is negligible. With escalating E: T (effector: target) ratio changes in the percentage of apoptotic cells in PB samples were significantly higher than CB samples. The mechanism( s) of the low cytotoxicity of resting cord NK cells is not welllmderstood. Complementary research in this field is recognized to elucidate the phenotypical and functional properties of CB cells and how they relate to maturational stages. CB studies are important for transplantation research and may provide insight to the suppressive mechanism by which the host -recipient could evade GVHD and rejection.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله m خیراندیش | m kheirandish
from the department of immunology school of medical sciences, tarbiat modalres university, tehran, and the iranian blood transfusion research center, tehran, iran.

سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)

m ابتکار | m ebtekar
from the department o lmll1l1n% gy school o medical sciences, tarbiat modaltes university, tehran, lnd the [ranian

سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)

aa پورفتح اله | aa pourfathollah
from the department o lmll1l1n% gy school o medical sciences, tarbiat modaltes university, tehran, lnd the [ranian

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

zm حسن | zm hassan
from the department o lmll1l1n% gy school o medical sciences, tarbiat modaltes university, tehran, lnd the [ranian

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

a کاظم نژاد | a kazem nejad
from the department o lmll1l1n% gy school o medical sciences, tarbiat modaltes university, tehran, lnd the [ranian

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-112&slc_lang=en&sid=en
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده 1
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات