این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Medical Journal of Islamic Republic of Iran، جلد ۱۷، شماره ۱، صفحات ۷۵-۷۹
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی COMPARISON OF VAL IDITY OF MICROLYMPHOCYTOTOXICITY AND FLOWCYTOMETRY METHODS WITH PCR FOR HL A-B27 ANTIGEN TYPING
چکیده انگلیسی مقاله The Human Major Histocompatibility Complex (MHC) plays a crucial role in transplantation, transfusion, paternity test and assessment of susceptibility to some diseases associated with HLA-B27. Three of the most fashionable methods for determination of HLA antigens in clinical and research laboratories are microlymphocytotoxicity (MLCT), flowcytometry and polymerase chain reaction (PCR). The purpose of this study was to compare the sensitivity, specificity, and positive and negative predictive values of MLCT and flowcytometry methods with PCR as a gold standard method in determination of HLA-B27 antigen. In the present study, all three above-mentioned techniques have been used for 36 patients suffering from ankylosing spondylitis and 31 healthy volunteers. Specific antisera and monoclonal antibodies against HLA-B27, and allele specific PCR have been used in MLCT, flowcytometry and PCR methods respectively. The results show that sensitivity, specificity, positive and negative predictive values of MLCT method as compared with PCR technique were 83.3%, 100%, 100% and 88.1 % respectively. Moreover, sensitivity, specificity, positive and negative predictive values of flowcytometry compared to the PCR technique were 100%, 94.6%, 93.8% and 100% respectively. Based on the results, the flowcytometry method in determination of HLAB27 is more valid than MLCT in this regard, particularly in research programs. The similarity between the results of our study and those studies done in Europe suggests the probability of resemblance between HLA-B27 subtypes in Europe and in Iran.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله mh نیکنام | mh nicknam
from the immunogenetics laboratory, immunology department, faculty of medicine, tehran university of medical sciences, tehran, i.r. iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

ar جمشیدی | ar jamshidi
from the immunogenetics laboratory, immunology department, faculty of medicine, tehran university of medical sciences, t

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

m گنجعلی خانی حاکمی | m ganjalikhani hakemi
from the immunogenetics laboratory, immunology department, faculty of medicine, tehran university of medical sciences, t

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

f خسروی | f khosravi
from the immunogenetics laboratory, immunology department, faculty of medicine, tehran university of medical sciences, t

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

a امیرخانی | a amirkhani
from the immunogenetics laboratory, immunology department, faculty of medicine, tehran university of medical sciences, t

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

m narouinejad | m narouinejad
from the immunogenetics laboratory, immunology department, faculty of medicine, tehran university of medical sciences, t

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

b نیک بین | b nikbin
from the immunogenetics laboratory, immunology department, faculty of medicine, tehran university of medical sciences, t

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-183&slc_lang=en&sid=en
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده 1
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات